Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F11%3A00359861" target="_blank" >RIV/61389013:_____/11:00359861 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.e-polymers.org/journal/abstract.cfm?abstract_Id=3616" target="_blank" >http://www.e-polymers.org/journal/abstract.cfm?abstract_Id=3616</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization

Popis výsledku v původním jazyce

Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they meet the most criteria for artificial tissue properties. To enhance the cell adhesion and proliferation, it is desirable tomodify the polymer surface by some bioactive compounds (e.g. saccharides, proteins). In this work, firstly carboxyl-enriched hydrogels were prepared by various methods: by copolymerization of 2 hydroxyethyl methacrylate with methacrylic acid, by hydrolysis or oxidation of poly(2 hydroxyethyl methacrylate). Subsequently, avidin was immobilized to the modified hydrogel surfaces either electrostatically through dissociated carboxylic groups or covalently through carboxylic groups activated by N-hydroxysuccinimide. Preliminary biological tests showed that hydrogels with immobilized avidin provides better adhesion and proliferation of keratinocytes compared to supports without avidin.

Název v anglickém jazyce

Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization

Popis výsledku anglicky

Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they meet the most criteria for artificial tissue properties. To enhance the cell adhesion and proliferation, it is desirable tomodify the polymer surface by some bioactive compounds (e.g. saccharides, proteins). In this work, firstly carboxyl-enriched hydrogels were prepared by various methods: by copolymerization of 2 hydroxyethyl methacrylate with methacrylic acid, by hydrolysis or oxidation of poly(2 hydroxyethyl methacrylate). Subsequently, avidin was immobilized to the modified hydrogel surfaces either electrostatically through dissociated carboxylic groups or covalently through carboxylic groups activated by N-hydroxysuccinimide. Preliminary biological tests showed that hydrogels with immobilized avidin provides better adhesion and proliferation of keratinocytes compared to supports without avidin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

<a href="/cs/project/1QS400500558" target="_blank" >1QS400500558: Bioaktivní nosič pro kultivaci a transplantaci buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

E-Polymers

ISSN

1618-7229

e-ISSN

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Svazek periodika

-

Číslo periodika v rámci svazku

43

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

000290474600001

EID výsledku v databázi Scopus

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