E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F11%3A00362894" target="_blank" >RIV/61389013:_____/11:00362894 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.3109/1061186X.2010.547585" target="_blank" >http://dx.doi.org/10.3109/1061186X.2010.547585</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/1061186X.2010.547585" target="_blank" >10.3109/1061186X.2010.547585</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
Popis výsledku v původním jazyce
Retargeting of adenovirus via E-selectin as a viable pathway of infection in tumor necrosis factor-? (TNF-?)-activated human umbilical vein endothelial cells (HUVECs) was investigated. E1, E3-deleted Ad5 expressing cytomegalovirus immediate-early promoter-driven luciferase (Adluc) was coated with a reactive multivalent copolymer based on poly [N-(2-hydroxypropyl) methacrylamide] to generate pHPMA-adenovirus (pcAdluc). This was then retargeted by covalent attachment of a mouse antihuman E-selectin monoclonal antibody (MHES mAb). MHESpcAdluc was efficiently taken up into HUVECs, generating a high level of transduction in TNF-?-treated E-selectin positive cells but not in untreated receptor-negative cells. Our results suggest that E-selectin could be a valuable target for gene transfer strategies internalizing polymer-coated adenovirus particles through a viable receptor-mediated endocytosis pathway, generating adequate levels of transgene expression per virus genome copy.
Název v anglickém jazyce
E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
Popis výsledku anglicky
Retargeting of adenovirus via E-selectin as a viable pathway of infection in tumor necrosis factor-? (TNF-?)-activated human umbilical vein endothelial cells (HUVECs) was investigated. E1, E3-deleted Ad5 expressing cytomegalovirus immediate-early promoter-driven luciferase (Adluc) was coated with a reactive multivalent copolymer based on poly [N-(2-hydroxypropyl) methacrylamide] to generate pHPMA-adenovirus (pcAdluc). This was then retargeted by covalent attachment of a mouse antihuman E-selectin monoclonal antibody (MHES mAb). MHESpcAdluc was efficiently taken up into HUVECs, generating a high level of transduction in TNF-?-treated E-selectin positive cells but not in untreated receptor-negative cells. Our results suggest that E-selectin could be a valuable target for gene transfer strategies internalizing polymer-coated adenovirus particles through a viable receptor-mediated endocytosis pathway, generating adequate levels of transgene expression per virus genome copy.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CD - Makromolekulární chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Drug Targeting
ISSN
1061-186X
e-ISSN
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Svazek periodika
19
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
690-700
Kód UT WoS článku
000293743800012
EID výsledku v databázi Scopus
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