SAXS study of sterically stabilized lipid nanocarriers functionalized by DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00376686" target="_blank" >RIV/61389013:_____/12:00376686 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1088/1742-6596/351/1/012004" target="_blank" >http://dx.doi.org/10.1088/1742-6596/351/1/012004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1742-6596/351/1/012004" target="_blank" >10.1088/1742-6596/351/1/012004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SAXS study of sterically stabilized lipid nanocarriers functionalized by DNA

Popis výsledku v původním jazyce

The structure of novel spontaneously self-assembled plasmid DNA/lipid complexes is investigated by means of small-angle X-ray scattering (SAXS) and Cryo-TEM imaging. Liquid crystalline (LC) hydrated lipid systems are prepared using the nonionic lipids monoolein and DOPE-PEG2000 and the cationic amphiphile CTAB. The employed plasmid DNA (pDNA) is encoding for the human protein brain-derived neurotrophic factor (BDNF). A coexistence of nanoparticulate objects with different LC inner organizations is established. A transition from bicontinuous membrane sponges, cubosome intermediates and unilamelar liposomes to multilamellar vesicles, functionalized by pDNA, is favoured upon binding and compaction of pBDNF onto the cationic PEGylated lipid nanocarriers. The obtained sterically stabilized nanovehicles, with confined supercoiled plasmid DNA (pBDNF), are important in the context of multicompartment lipid nanocarriers of interest for gene therapy of neurodegenerative diseases.

Název v anglickém jazyce

SAXS study of sterically stabilized lipid nanocarriers functionalized by DNA

Popis výsledku anglicky

The structure of novel spontaneously self-assembled plasmid DNA/lipid complexes is investigated by means of small-angle X-ray scattering (SAXS) and Cryo-TEM imaging. Liquid crystalline (LC) hydrated lipid systems are prepared using the nonionic lipids monoolein and DOPE-PEG2000 and the cationic amphiphile CTAB. The employed plasmid DNA (pDNA) is encoding for the human protein brain-derived neurotrophic factor (BDNF). A coexistence of nanoparticulate objects with different LC inner organizations is established. A transition from bicontinuous membrane sponges, cubosome intermediates and unilamelar liposomes to multilamellar vesicles, functionalized by pDNA, is favoured upon binding and compaction of pBDNF onto the cationic PEGylated lipid nanocarriers. The obtained sterically stabilized nanovehicles, with confined supercoiled plasmid DNA (pBDNF), are important in the context of multicompartment lipid nanocarriers of interest for gene therapy of neurodegenerative diseases.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physics: Conference Series

ISSN

1742-6588

e-ISSN

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Svazek periodika

351

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000308218400004

EID výsledku v databázi Scopus

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