Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F14%3A00399047" target="_blank" >RIV/61389013:_____/14:00399047 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2013.09.009" target="_blank" >http://dx.doi.org/10.1016/j.msec.2013.09.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2013.09.009" target="_blank" >10.1016/j.msec.2013.09.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups

Popis výsledku v původním jazyce

Magnetic poly(glycidyl methacrylate)-based macroporous microspheres with an average particle size of 4.2 ?m were prepared using a modified multi-step swelling polymerization method and by introducing amino functionality on their surfaces. Antibody molecules were oxidized on their carbohydrate moieties and bound to the amino-containing magnetic microspheres via a site-directed procedure. CD133-positive cells could be effectively captured from human cancer cell lines (HepG2, HCT116, MCF7, and IMR-32) by using magnetic microspheres conjugated to an anti-human CD133 antibody. After further culture, the immunocaptured CD133-expressing cells from IMR-32 proliferated and gradually detached from the magnetic microspheres. Flow-cytometric analysis confirmed theenrichment of CD133-expressing cells by using the antibody-bound magnetic microspheres. Such microspheres suitable for immunocapture are very promising for cancer diagnosis because the CD133-expressing cells in cancer cell lines have bee

Název v anglickém jazyce

Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups

Popis výsledku anglicky

Magnetic poly(glycidyl methacrylate)-based macroporous microspheres with an average particle size of 4.2 ?m were prepared using a modified multi-step swelling polymerization method and by introducing amino functionality on their surfaces. Antibody molecules were oxidized on their carbohydrate moieties and bound to the amino-containing magnetic microspheres via a site-directed procedure. CD133-positive cells could be effectively captured from human cancer cell lines (HepG2, HCT116, MCF7, and IMR-32) by using magnetic microspheres conjugated to an anti-human CD133 antibody. After further culture, the immunocaptured CD133-expressing cells from IMR-32 proliferated and gradually detached from the magnetic microspheres. Flow-cytometric analysis confirmed theenrichment of CD133-expressing cells by using the antibody-bound magnetic microspheres. Such microspheres suitable for immunocapture are very promising for cancer diagnosis because the CD133-expressing cells in cancer cell lines have bee

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GCP207%2F12%2FJ013" target="_blank" >GCP207/12/J013: Inovativní multifunkční magnetické nano- a mikročástice pro buněčnou diagnostiku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

1 January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

193-200

Kód UT WoS článku

000330489500024

EID výsledku v databázi Scopus

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