Poly(ethylene oxide) brushes prepared by the "grafting to" method as a platform for the assessment of cell receptor-ligand binding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F14%3A00429250" target="_blank" >RIV/61389013:_____/14:00429250 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/14:00429250

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2014.06.004" target="_blank" >http://dx.doi.org/10.1016/j.eurpolymj.2014.06.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2014.06.004" target="_blank" >10.1016/j.eurpolymj.2014.06.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Poly(ethylene oxide) brushes prepared by the "grafting to" method as a platform for the assessment of cell receptor-ligand binding

Popis výsledku v původním jazyce

Poly(ethylene oxide) (PEO) with terminal alkyne and amino groups was grafted to a poly(glycidyl methacrylate) (PGMA) anchoring layer and the PEO/PGMA coatings were investigated as a non-fouling platform for the assessment of ligand?cell receptor interactions. The PEO/PGMA coatings deposited on Si/SiO2 substrate were stable in phosphate buffered saline over a period of 8 days if the thickness of the PEO was less than 30 nm. The stability of the coating could be enhanced by an additional layer of 3-mercaptopropyltrimethoxysilane between the substrate and the PGMA layer. The grafted layers were shown to efficiently suppress nonspecific protein adsorption and cell adhesion. Based on the theoretical ligand-binding capacity, protein adsorption and stabilitydata, the optimum thickness range of the PEO layer is 10?20 nm. The binding of an arginine?glycine?aspartic acid (RGD) ligand using azide?alkyne click chemistry demonstrated that the ligand surface density is controllable in the range fro

Název v anglickém jazyce

Poly(ethylene oxide) brushes prepared by the "grafting to" method as a platform for the assessment of cell receptor-ligand binding

Popis výsledku anglicky

Poly(ethylene oxide) (PEO) with terminal alkyne and amino groups was grafted to a poly(glycidyl methacrylate) (PGMA) anchoring layer and the PEO/PGMA coatings were investigated as a non-fouling platform for the assessment of ligand?cell receptor interactions. The PEO/PGMA coatings deposited on Si/SiO2 substrate were stable in phosphate buffered saline over a period of 8 days if the thickness of the PEO was less than 30 nm. The stability of the coating could be enhanced by an additional layer of 3-mercaptopropyltrimethoxysilane between the substrate and the PGMA layer. The grafted layers were shown to efficiently suppress nonspecific protein adsorption and cell adhesion. Based on the theoretical ligand-binding capacity, protein adsorption and stabilitydata, the optimum thickness range of the PEO layer is 10?20 nm. The binding of an arginine?glycine?aspartic acid (RGD) ligand using azide?alkyne click chemistry demonstrated that the ligand surface density is controllable in the range fro

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

11-22

Kód UT WoS článku

000341675400002

EID výsledku v databázi Scopus

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