Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00443253" target="_blank" >RIV/61389013:_____/15:00443253 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/14:PU108364 RIV/00216305:26310/15:PU119431

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fbp.2014.05.001" target="_blank" >http://dx.doi.org/10.1016/j.fbp.2014.05.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fbp.2014.05.001" target="_blank" >10.1016/j.fbp.2014.05.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

Popis výsledku v původním jazyce

Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6000 concentration ranging from 5.5 to 8%. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) – P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16% PEG 6000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of microspheres were tested for DNA isolation from crude cell lysates of different dairy products in solutions of 16% PEG 6000 and 2.0 M NaCl. Isolated DNAs were without PCR inhibitors, which was demonstrated using real-time PCR including melting analysis of the amplicons.

Název v anglickém jazyce

Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

Popis výsledku anglicky

Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6000 concentration ranging from 5.5 to 8%. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) – P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16% PEG 6000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of microspheres were tested for DNA isolation from crude cell lysates of different dairy products in solutions of 16% PEG 6000 and 2.0 M NaCl. Isolated DNAs were without PCR inhibitors, which was demonstrated using real-time PCR including melting analysis of the amplicons.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Bioproducts Processing

ISSN

0960-3085

e-ISSN

—

Svazek periodika

94

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

375-381

Kód UT WoS článku

000354140500039

EID výsledku v databázi Scopus

2-s2.0-84927575286