Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00445033" target="_blank" >RIV/61389013:_____/15:00445033 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >http://dx.doi.org/10.1016/j.ab.2015.04.037</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >10.1016/j.ab.2015.04.037</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Popis výsledku v původním jazyce

Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbuminantibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-

Název v anglickém jazyce

Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Popis výsledku anglicky

Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbuminantibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP206%2F12%2F0381" target="_blank" >GAP206/12/0381: Vývoj ultrasensitivních elektrochemických imunosenzorů pro včasnou detekci klinicky významných biomarkerů maligních onemocnění</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

—

Svazek periodika

484

Číslo periodika v rámci svazku

1 September

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

4-8

Kód UT WoS článku

000357967500002

EID výsledku v databázi Scopus

2-s2.0-84934969110