Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00445996" target="_blank" >RIV/61389013:_____/15:00445996 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/C5RA09280A" target="_blank" >http://dx.doi.org/10.1039/C5RA09280A</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C5RA09280A" target="_blank" >10.1039/C5RA09280A</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Popis výsledku v původním jazyce

Systemic lupus erythematosus (SLE) is a heterogeneous, inflammatory and multisystem autoimmune disease in which antinuclear antibodies are present in blood often years before clinical symptoms occur. Isolating the antibodies is thus of crucial importanceto confirm the diagnosis and prognosis of patients with some autoimmune diseases. Isolation can be performed advantageously using magnetic microspheres, which offer easy and quick manipulation with a magnet and avoid sample dilution. Here, we developedcalf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) (POEMA?His) microspheres using a multiple-stage swelling technique followed by His immobilization. Magnetic POEMA?His microspheres were characterized using scanning and transmission electron microscopy, SQUID, ATR FT-IR spectroscopy, elemental analysis and atomic absorption spectrometry. The microspheres were successfully used for rapid purification of the anti-histone immunoglobulins (IgGs) from blood serum samples of

Název v anglickém jazyce

Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Popis výsledku anglicky

Systemic lupus erythematosus (SLE) is a heterogeneous, inflammatory and multisystem autoimmune disease in which antinuclear antibodies are present in blood often years before clinical symptoms occur. Isolating the antibodies is thus of crucial importanceto confirm the diagnosis and prognosis of patients with some autoimmune diseases. Isolation can be performed advantageously using magnetic microspheres, which offer easy and quick manipulation with a magnet and avoid sample dilution. Here, we developedcalf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) (POEMA?His) microspheres using a multiple-stage swelling technique followed by His immobilization. Magnetic POEMA?His microspheres were characterized using scanning and transmission electron microscopy, SQUID, ATR FT-IR spectroscopy, elemental analysis and atomic absorption spectrometry. The microspheres were successfully used for rapid purification of the anti-histone immunoglobulins (IgGs) from blood serum samples of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/7E12053" target="_blank" >7E12053: Nanosystems for early Diagnosis of Neurodegenerative Diseases</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

77

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

63050-63055

Kód UT WoS článku

000358620100089

EID výsledku v databázi Scopus

2-s2.0-84938372716