Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00450673" target="_blank" >RIV/61389013:_____/15:00450673 - isvavai.cz</a>

Výsledek na webu

<a href="http://joam.inoe.ro/index.php?option=magazine&op=view&idu=3833&catid=92" target="_blank" >http://joam.inoe.ro/index.php?option=magazine&op=view&idu=3833&catid=92</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration

Popis výsledku v původním jazyce

The results of small angle scattering investigation of protein apoferritin are presented. In particular, the results of approximation to zero value of concentration are calculated. The procedure of approximating at zero concentration of apoferritin in this work is considered. The sizes and shapes, including those determined by indirect Fourier transform method, obtained on different instruments are compared. The pair-distance distribution function for both small angle neutron (SANS) and X-ray scattering(SAXS) is computed. It is shown that SANS and SAXS methods give similar form factor (spherical shell with holes) of apoferritin. At the same time, fits of experimental data for SAXS and SANS curves give different sizes and volumes of the molecule. The reasons for these discrepancies are discussed.

Název v anglickém jazyce

Low resolution structural studies of apoferritin via SANS and SAXS: the effect of concentration

Popis výsledku anglicky

The results of small angle scattering investigation of protein apoferritin are presented. In particular, the results of approximation to zero value of concentration are calculated. The procedure of approximating at zero concentration of apoferritin in this work is considered. The sizes and shapes, including those determined by indirect Fourier transform method, obtained on different instruments are compared. The pair-distance distribution function for both small angle neutron (SANS) and X-ray scattering(SAXS) is computed. It is shown that SANS and SAXS methods give similar form factor (spherical shell with holes) of apoferritin. At the same time, fits of experimental data for SAXS and SANS curves give different sizes and volumes of the molecule. The reasons for these discrepancies are discussed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Optoelectronics and Advanced Materials

ISSN

1454-4164

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

9-10

Stát vydavatele periodika

RO - Rumunsko

Počet stran výsledku

6

Strana od-do

1397-1402

Kód UT WoS článku

000364600400027

EID výsledku v databázi Scopus

2-s2.0-84953402029