Differential scanning fluorimetry illuminates silk feedstock stability and processability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00452866" target="_blank" >RIV/61389013:_____/16:00452866 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/C5SM02036k" target="_blank" >http://dx.doi.org/10.1039/C5SM02036k</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C5SM02036k" target="_blank" >10.1039/C5SM02036k</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential scanning fluorimetry illuminates silk feedstock stability and processability

Popis výsledku v původním jazyce

The ability to design and implement silk feedstock formulations for tailored spinning has so far eluded the bioengineers. Recently, the high throughput screening technique of differential scanning fluorimetry (DSF) demonstrated the link between the instability transition temperature (Ti) and the processability of the silk feedstock. Using DSF we screened a large set of chemicals known to affect solvent quality. A multivariate analysis of the results shows that, regardless of the diversity of chemicals,three groupings are significantly distinguishable: G1 = similar to native silk; G2 = largely dominated by electrostatic interactions; and G3 = dominated by chelating interactions. We propose a thermodynamic analysis based on a pre- and post-transition fit to estimate the van't Hoff enthalpies (?Hv) and the instability temperature (Ti). Our analysis shows that the ?Ti and ?Hv values were distinct: G1 (?Ti = 0.23 0.2; ?Hv = 159.1 5.6 kcal mol1), G2 (?Ti = 7.3 0.7; ?Hv = 191.4 5.5 kcal mol1

Název v anglickém jazyce

Differential scanning fluorimetry illuminates silk feedstock stability and processability

Popis výsledku anglicky

The ability to design and implement silk feedstock formulations for tailored spinning has so far eluded the bioengineers. Recently, the high throughput screening technique of differential scanning fluorimetry (DSF) demonstrated the link between the instability transition temperature (Ti) and the processability of the silk feedstock. Using DSF we screened a large set of chemicals known to affect solvent quality. A multivariate analysis of the results shows that, regardless of the diversity of chemicals,three groupings are significantly distinguishable: G1 = similar to native silk; G2 = largely dominated by electrostatic interactions; and G3 = dominated by chelating interactions. We propose a thermodynamic analysis based on a pre- and post-transition fit to estimate the van't Hoff enthalpies (?Hv) and the instability temperature (Ti). Our analysis shows that the ?Ti and ?Hv values were distinct: G1 (?Ti = 0.23 0.2; ?Hv = 159.1 5.6 kcal mol1), G2 (?Ti = 7.3 0.7; ?Hv = 191.4 5.5 kcal mol1

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/EE2.3.30.0029" target="_blank" >EE2.3.30.0029: BIOPOL: BIOpolymerní POstdoktorandská Laboratoř a vzdělávácí centrum</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soft Matter

ISSN

1744-683X

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

255-262

Kód UT WoS článku

000366863800030

EID výsledku v databázi Scopus

2-s2.0-84951130318