RAFT polymerization of N,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00457808" target="_blank" >RIV/61389013:_____/16:00457808 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/pola.27939" target="_blank" >http://dx.doi.org/10.1002/pola.27939</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pola.27939" target="_blank" >10.1002/pola.27939</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RAFT polymerization of N,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

Popis výsledku v původním jazyce

Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N-dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres, 4 µm in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2-(methacryloyl)oxyethyl acetate (HEMA-Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide-alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible additionfragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions.

Název v anglickém jazyce

RAFT polymerization of N,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

Popis výsledku anglicky

Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N-dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres, 4 µm in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2-(methacryloyl)oxyethyl acetate (HEMA-Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide-alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible additionfragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/LH14318" target="_blank" >LH14318: Syntéza polymerem povlečených magnetických nanočástic pro biologii a lékařství</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Polymer Science. Part A - Polymer Chemistry

ISSN

0887-624X

e-ISSN

—

Svazek periodika

54

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1036-1043

Kód UT WoS článku

000371890200002

EID výsledku v databázi Scopus

2-s2.0-84960436071