System with embedded drug release and nanoparticle degradation sensor showing efficient rifampicin delivery into macrophages

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00470718" target="_blank" >RIV/61389013:_____/17:00470718 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/17:10360758 RIV/00216208:11130/17:10360758 RIV/00064203:_____/17:10360758

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.nano.2016.08.031" target="_blank" >http://dx.doi.org/10.1016/j.nano.2016.08.031</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nano.2016.08.031" target="_blank" >10.1016/j.nano.2016.08.031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

System with embedded drug release and nanoparticle degradation sensor showing efficient rifampicin delivery into macrophages

Popis výsledku v původním jazyce

We have developed a biodegradable, biocompatible system for the delivery of the antituberculotic antibiotic rifampicin with a built-in drug release and nanoparticle degradation fluorescence sensor. Polymer nanoparticles based on poly(ethylene oxide) monomethyl ether-block-poly(epsilon-caprolactone) were noncovalently loaded with rifampicin, a combination that, to best of our knowledge, was not previously described in the literature, which showed significant benefits. The nanoparticles contain a Förster resonance energy transfer (FRET) system that allows real-time assessment of drug release not only in vitro, but also in living macrophages where the mycobacteria typically reside as hard-to-kill intracellular parasites. The fluorophore also enables in situ monitoring of the enzymatic nanoparticle degradation in the macrophages. We show that the nanoparticles are efficiently taken up by macrophages, where they are very quickly associated with the lysosomal compartment. After drug release, the nanoparticles in the cmacrophages are enzymatically degraded, with half-life 88 ± 11 min.

Název v anglickém jazyce

System with embedded drug release and nanoparticle degradation sensor showing efficient rifampicin delivery into macrophages

Popis výsledku anglicky

We have developed a biodegradable, biocompatible system for the delivery of the antituberculotic antibiotic rifampicin with a built-in drug release and nanoparticle degradation fluorescence sensor. Polymer nanoparticles based on poly(ethylene oxide) monomethyl ether-block-poly(epsilon-caprolactone) were noncovalently loaded with rifampicin, a combination that, to best of our knowledge, was not previously described in the literature, which showed significant benefits. The nanoparticles contain a Förster resonance energy transfer (FRET) system that allows real-time assessment of drug release not only in vitro, but also in living macrophages where the mycobacteria typically reside as hard-to-kill intracellular parasites. The fluorophore also enables in situ monitoring of the enzymatic nanoparticle degradation in the macrophages. We show that the nanoparticles are efficiently taken up by macrophages, where they are very quickly associated with the lysosomal compartment. After drug release, the nanoparticles in the cmacrophages are enzymatically degraded, with half-life 88 ± 11 min.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanomedicine: Nanotechnology, Biology and Medicine

ISSN

1549-9634

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

307-315

Kód UT WoS článku

000396378200028

EID výsledku v databázi Scopus

2-s2.0-84998881945