Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00472812" target="_blank" >RIV/61389013:_____/17:00472812 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/term.1982" target="_blank" >http://dx.doi.org/10.1002/term.1982</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/term.1982" target="_blank" >10.1002/term.1982</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

Popis výsledku v původním jazyce

In this study, fibrous scaffolds based on poly(gamma-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20–400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres’ surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units. Second, modification with the short peptide sequence azidopentanoyl–GGGRGDSGGGY–NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10–3–10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs.

Název v anglickém jazyce

Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering

Popis výsledku anglicky

In this study, fibrous scaffolds based on poly(gamma-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20–400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres’ surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units. Second, modification with the short peptide sequence azidopentanoyl–GGGRGDSGGGY–NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10–3–10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Tissue Engineering and Regenerative Medicine

ISSN

1932-6254

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

831-842

Kód UT WoS článku

000398046600023

EID výsledku v databázi Scopus

2-s2.0-84921534324