Carbon nanospecies affecting amyloid formation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00481916" target="_blank" >RIV/61389013:_____/17:00481916 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10368179

Výsledek na webu

<a href="http://dx.doi.org/10.1039/C7RA11296C" target="_blank" >http://dx.doi.org/10.1039/C7RA11296C</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C7RA11296C" target="_blank" >10.1039/C7RA11296C</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carbon nanospecies affecting amyloid formation

Popis výsledku v původním jazyce

Carbon nanospecies (CNPs) are of high interest in current research due to their many unique properties. They may be created by common processes, such as burning. Therefore, they can become potential contaminants and may have a negative impact on human health and the biosphere. Moreover, they may also catalyze protein misfolding and subsequent amyloid formation, which is extremely hard to treat. We investigated the influence of single-walled carbon nanotubes (SWNTs), fullerene (C60), carbon quantum dots (CDs) and nanodiamonds (NDs) on amyloid formation. This research utilized the hen egg-white lysozyme (HEWL) as a model system. Fibrils were detected by fluorescence of thioflavin-T (ThT) or Nile red (NR) and the results were confirmed by transmission electron microscopy (TEM). We have found that NDs promoted amyloid fibril formation at all concentrations. The highest concentration of C60 (250 µg ml-1) accelerated the process of fibrillation, while smaller concentrations (16 and 80 µg ml-1) prolonged the lag phase and were comparable to the control. SWNTs prolonged the lag phase of amyloid formation at all concentrations. CDs efficiently terminated the growth of amyloid fibrils. When we compared the amyloidogenicity of all four types of CNPs, the following trend was apparent: NDs > control > C60 > CDs > SWNTs.

Název v anglickém jazyce

Carbon nanospecies affecting amyloid formation

Popis výsledku anglicky

Carbon nanospecies (CNPs) are of high interest in current research due to their many unique properties. They may be created by common processes, such as burning. Therefore, they can become potential contaminants and may have a negative impact on human health and the biosphere. Moreover, they may also catalyze protein misfolding and subsequent amyloid formation, which is extremely hard to treat. We investigated the influence of single-walled carbon nanotubes (SWNTs), fullerene (C60), carbon quantum dots (CDs) and nanodiamonds (NDs) on amyloid formation. This research utilized the hen egg-white lysozyme (HEWL) as a model system. Fibrils were detected by fluorescence of thioflavin-T (ThT) or Nile red (NR) and the results were confirmed by transmission electron microscopy (TEM). We have found that NDs promoted amyloid fibril formation at all concentrations. The highest concentration of C60 (250 µg ml-1) accelerated the process of fibrillation, while smaller concentrations (16 and 80 µg ml-1) prolonged the lag phase and were comparable to the control. SWNTs prolonged the lag phase of amyloid formation at all concentrations. CDs efficiently terminated the growth of amyloid fibrils. When we compared the amyloidogenicity of all four types of CNPs, the following trend was apparent: NDs > control > C60 > CDs > SWNTs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

85

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

53887-53898

Kód UT WoS článku

000416831000028

EID výsledku v databázi Scopus

2-s2.0-85035363583