Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00489135" target="_blank" >RIV/61389013:_____/18:00489135 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acs.macromol.7b02158" target="_blank" >http://dx.doi.org/10.1021/acs.macromol.7b02158</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.macromol.7b02158" target="_blank" >10.1021/acs.macromol.7b02158</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy

Popis výsledku v původním jazyce

Fluorescence correlation spectroscopy (FCS) provides most commonly values of diffusion coefficients of fluorescently labeled species in a solution, especially biopolymers. A newly developed procedure for the determination of diffusion coefficient distributions applicable to polydisperse polymers or nanoparticles, based on the well-known CONTIN algorithm, is described and tested on both simulated FCS correlation functions and real experimental data. Good resolution of bimodal distributions is observed, and it is quantitatively established how the resolution depends on the level of experimental noise. Effects of incorrect calibration of the focal volume on the obtained diffusion coefficient and its distribution are described for single-focus FCS. With rapid expansion of the FCS method from biology and biochemistry into the polymer field, and recently into many other disciplines including, e.g., environmental sciences, such a technique was very much needed.

Název v anglickém jazyce

Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy

Popis výsledku anglicky

Fluorescence correlation spectroscopy (FCS) provides most commonly values of diffusion coefficients of fluorescently labeled species in a solution, especially biopolymers. A newly developed procedure for the determination of diffusion coefficient distributions applicable to polydisperse polymers or nanoparticles, based on the well-known CONTIN algorithm, is described and tested on both simulated FCS correlation functions and real experimental data. Good resolution of bimodal distributions is observed, and it is quantitatively established how the resolution depends on the level of experimental noise. Effects of incorrect calibration of the focal volume on the obtained diffusion coefficient and its distribution are described for single-focus FCS. With rapid expansion of the FCS method from biology and biochemistry into the polymer field, and recently into many other disciplines including, e.g., environmental sciences, such a technique was very much needed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/LO1507" target="_blank" >LO1507: Polymery pro pokročilé technologie i kvalitnější život</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macromolecules

ISSN

0024-9297

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

2796-2804

Kód UT WoS článku

000431088700005

EID výsledku v databázi Scopus

2-s2.0-85045895401