Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00490445" target="_blank" >RIV/61389013:_____/18:00490445 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00396-018-4329-4" target="_blank" >http://dx.doi.org/10.1007/s00396-018-4329-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00396-018-4329-4" target="_blank" >10.1007/s00396-018-4329-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC

Popis výsledku v původním jazyce

The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.

Název v anglickém jazyce

Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC

Popis výsledku anglicky

The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/LH15213" target="_blank" >LH15213: Chování a tvar nanočástic – protirakovinných nosičů léčiv ve skutečném prostředí krve.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloid and Polymer Science

ISSN

0303-402X

e-ISSN

—

Svazek periodika

296

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1183-1191

Kód UT WoS článku

000435592300009

EID výsledku v databázi Scopus

2-s2.0-85047362735