Antioxidant polymer-modified maghemite nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00496525" target="_blank" >RIV/61389013:_____/19:00496525 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0304885318317438?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0304885318317438?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jmmm.2018.10.081" target="_blank" >10.1016/j.jmmm.2018.10.081</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antioxidant polymer-modified maghemite nanoparticles

Popis výsledku v původním jazyce

Natural antioxidants, such as epigallocatechin-3-gallate and related phenolic compounds from tea, enhance particle cell-interactions and cellular uptake. In this study, surface of superparamagnetic iron oxide nanoparticles prepared by co-precipitation of Fe chlorides was modified with silica, polyethylenimine, poly(ethylene glycol), and poly(l-lysine) to protect the iron oxide core from redox-reactions with phenols, enhance uptake by the cells, prevent the particle aggregation, or enable conjugation with several phenol-based antioxidants. To reveal the relation between the particle uptake and chemical structure of the phenolic antioxidants, five of them were selected, namely phenol, phloroglucinol, chlorogenic, gallic, and tannic acid. After incubation of the phenol-modified nanoparticles with U87MG human glioma cells, intracellular levels of the reactive oxygen species were reduced in a similar manner, as measured by flow cytometry. Colorimetric iron assay revealed a comparable level of cell-associated particles, which was mostly independent on the applied external magnetic field. The results suggest that the poly(l-lysine)-based coating is responsible for the antioxidant effects of the particles. The phenol- and poly(l-lysine)-coatings enable effective colloidal stability in aqueous media and enhance cellular internalization of the nanoparticles.

Název v anglickém jazyce

Antioxidant polymer-modified maghemite nanoparticles

Popis výsledku anglicky

Natural antioxidants, such as epigallocatechin-3-gallate and related phenolic compounds from tea, enhance particle cell-interactions and cellular uptake. In this study, surface of superparamagnetic iron oxide nanoparticles prepared by co-precipitation of Fe chlorides was modified with silica, polyethylenimine, poly(ethylene glycol), and poly(l-lysine) to protect the iron oxide core from redox-reactions with phenols, enhance uptake by the cells, prevent the particle aggregation, or enable conjugation with several phenol-based antioxidants. To reveal the relation between the particle uptake and chemical structure of the phenolic antioxidants, five of them were selected, namely phenol, phloroglucinol, chlorogenic, gallic, and tannic acid. After incubation of the phenol-modified nanoparticles with U87MG human glioma cells, intracellular levels of the reactive oxygen species were reduced in a similar manner, as measured by flow cytometry. Colorimetric iron assay revealed a comparable level of cell-associated particles, which was mostly independent on the applied external magnetic field. The results suggest that the poly(l-lysine)-based coating is responsible for the antioxidant effects of the particles. The phenol- and poly(l-lysine)-coatings enable effective colloidal stability in aqueous media and enhance cellular internalization of the nanoparticles.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GC16-01128J" target="_blank" >GC16-01128J: Magnetické nanočástice s antioxidačním účinkem na bázi přírodních antioxidantů: Interakce nanočástic s buňkami</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Magnetism and Magnetic Materials

ISSN

0304-8853

e-ISSN

—

Svazek periodika

473

Číslo periodika v rámci svazku

1 March

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

517-526

Kód UT WoS článku

000450580900081

EID výsledku v databázi Scopus

2-s2.0-85056468814