Zwitterionic functionalizable scaffolds with gyroid pore architecture for tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F19%3A00504008" target="_blank" >RIV/61389013:_____/19:00504008 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201800403" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201800403</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mabi.201800403" target="_blank" >10.1002/mabi.201800403</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Zwitterionic functionalizable scaffolds with gyroid pore architecture for tissue engineering

Popis výsledku v původním jazyce

Stereolithography‐assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω‐methacrylate poly(d,l‐lactide‐block‐ethylene glycol‐block‐ d,l‐lactide) (MA‐PDLLA‐PEG‐PDLLA‐MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X‐ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.

Název v anglickém jazyce

Zwitterionic functionalizable scaffolds with gyroid pore architecture for tissue engineering

Popis výsledku anglicky

Stereolithography‐assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω‐methacrylate poly(d,l‐lactide‐block‐ethylene glycol‐block‐ d,l‐lactide) (MA‐PDLLA‐PEG‐PDLLA‐MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X‐ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macromolecular Bioscience

ISSN

1616-5187

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000465021800002

EID výsledku v databázi Scopus

2-s2.0-85060123101