A 2D-Raman correlation spectroscopy study of the interaction of the polymer nanocomposites with carbon nanotubes and human osteoblast-like cells interface

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F20%3A00523879" target="_blank" >RIV/61389013:_____/20:00523879 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0022286020304609?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022286020304609?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molstruc.2020.128135" target="_blank" >10.1016/j.molstruc.2020.128135</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A 2D-Raman correlation spectroscopy study of the interaction of the polymer nanocomposites with carbon nanotubes and human osteoblast-like cells interface

Popis výsledku v původním jazyce

The objects of this research were two types of polymer nanocomposites: poly(ε-caprolactone)/multiwalled carbon nanotubes (PCL/MWCNTs) and poly(ε-caprolactone)/functionalized multiwalled carbon nanotubes (PCL/MWCNTs-f) (without and with oxidative treatment of MWCNTs prior to nanocomposite production, respectively). These nanocomposites are promising materials for medical and tissue engineering applications. The cell proliferation test, performed by culturing of osteoblast-like U-2 OS cells line (human bone osteosarcoma epithelial cells) on the surface of tested nanocomposites, confirmed their high biocompatibility. In order to distinguish the nature of cells-material interactions the two-dimensional (2D) Raman correlation spectroscopy technique was used. It revealed more developed changes in the proteins of the cells cultured on the PCL/MWCNTs-f compare to PCL/MWCNTs, as the conformation β of amide I emerged in the asynchronous map of PCL/MWCNTs-f. Moreover, it was confirmed that the functionalization of MWCNTs leads to a different pattern of nanocomposite interaction with cells, for PCL/MWCNTs-f the formed cross-peaks indicate the creation of combined domains of polymer with cell proteins. Whereas for PCL/MWCNTs it was predominantly the nanotubes’ amorphous component D1 that induced the cross-peaks formation.

Název v anglickém jazyce

A 2D-Raman correlation spectroscopy study of the interaction of the polymer nanocomposites with carbon nanotubes and human osteoblast-like cells interface

Popis výsledku anglicky

The objects of this research were two types of polymer nanocomposites: poly(ε-caprolactone)/multiwalled carbon nanotubes (PCL/MWCNTs) and poly(ε-caprolactone)/functionalized multiwalled carbon nanotubes (PCL/MWCNTs-f) (without and with oxidative treatment of MWCNTs prior to nanocomposite production, respectively). These nanocomposites are promising materials for medical and tissue engineering applications. The cell proliferation test, performed by culturing of osteoblast-like U-2 OS cells line (human bone osteosarcoma epithelial cells) on the surface of tested nanocomposites, confirmed their high biocompatibility. In order to distinguish the nature of cells-material interactions the two-dimensional (2D) Raman correlation spectroscopy technique was used. It revealed more developed changes in the proteins of the cells cultured on the PCL/MWCNTs-f compare to PCL/MWCNTs, as the conformation β of amide I emerged in the asynchronous map of PCL/MWCNTs-f. Moreover, it was confirmed that the functionalization of MWCNTs leads to a different pattern of nanocomposite interaction with cells, for PCL/MWCNTs-f the formed cross-peaks indicate the creation of combined domains of polymer with cell proteins. Whereas for PCL/MWCNTs it was predominantly the nanotubes’ amorphous component D1 that induced the cross-peaks formation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular Structure

ISSN

0022-2860

e-ISSN

—

Svazek periodika

1212

Číslo periodika v rámci svazku

15 July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000529345400006

EID výsledku v databázi Scopus

2-s2.0-85082864095