Сolloidal probe based on iron(III)-doped calcium phytate nanoparticles for 31P NMR monitoring of bacterial siderophores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F21%3A00542487" target="_blank" >RIV/61389013:_____/21:00542487 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2215038221000674?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2215038221000674?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colcom.2021.100427" target="_blank" >10.1016/j.colcom.2021.100427</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Сolloidal probe based on iron(III)-doped calcium phytate nanoparticles for 31P NMR monitoring of bacterial siderophores

Popis výsledku v původním jazyce

A conceptually new class of probes was developed for detection of bacterial siderophores (BSPs), important determinants of virulence and pathogenicity, using 31P nuclear magnetic resonance (31P NMR). These probes are based on biodegradable nontoxic nanoparticles of calcium phytate (CaIP6), which possess measurable signal on 31P NMR spectra. The nanoparticles were doped with paramagnetic Fe3+ ions broadening the magnetic resonance signal of 31P. Therefore, the nanoparticles were not detectable by 31P NMR in the environment not containing BSPs, however, in the presence of BSPs, the Fe3+ ion is released from the nanoparticles, and the 31P NMR signal become detectable. In vitro demonstration of this Fe3+ release was performed with BSP deferoxamine (DFO), exhibiting a complete depletion of Fe3+ within 2 h for the nanoparticles containing 17.7 mol% Fe (III). The Fe3+ depletion was accompanied with a restoration of 31P NMR signal.

Název v anglickém jazyce

Сolloidal probe based on iron(III)-doped calcium phytate nanoparticles for 31P NMR monitoring of bacterial siderophores

Popis výsledku anglicky

A conceptually new class of probes was developed for detection of bacterial siderophores (BSPs), important determinants of virulence and pathogenicity, using 31P nuclear magnetic resonance (31P NMR). These probes are based on biodegradable nontoxic nanoparticles of calcium phytate (CaIP6), which possess measurable signal on 31P NMR spectra. The nanoparticles were doped with paramagnetic Fe3+ ions broadening the magnetic resonance signal of 31P. Therefore, the nanoparticles were not detectable by 31P NMR in the environment not containing BSPs, however, in the presence of BSPs, the Fe3+ ion is released from the nanoparticles, and the 31P NMR signal become detectable. In vitro demonstration of this Fe3+ release was performed with BSP deferoxamine (DFO), exhibiting a complete depletion of Fe3+ within 2 h for the nanoparticles containing 17.7 mol% Fe (III). The Fe3+ depletion was accompanied with a restoration of 31P NMR signal.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloid and Interface Science Communications

ISSN

2215-0382

e-ISSN

2215-0382

Svazek periodika

42

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

100427

Kód UT WoS článku

000658652600007

EID výsledku v databázi Scopus

2-s2.0-85106305813