Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00552077" target="_blank" >RIV/61389013:_____/22:00552077 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023736:_____/22:00013332

Výsledek na webu

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262484" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262484</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0262484" target="_blank" >10.1371/journal.pone.0262484</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome

Popis výsledku v původním jazyce

Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hematological malignancies.Here, we investigated proteome changes in the exosome-rich fraction of the plasma of myelodysplastic syndrome patients and healthy donors. Exosome-rich fraction of the plasma was isolated using ExoQuick™: proteomes were compared and statistically processed, proteins were identified by nanoLC-MS/MS and verified using the ExoCarta and QuickGO databases. Mann-Whitney and Spearman analyses were used to statistically analyze the data. 2D western blot was used to monitor clusterin proteoforms. Statistical analyses of the data highlighted clusterin alterations as the most significant. 2D western blot showed that the clusterin changes were caused by posttranslational modifications. Moreover, there was a notable increase in the clusterin proteoform in the exosome-rich fraction of plasma of patients with more severe myelodysplastic syndrome, this corresponded with a simultaneous decrease in their plasma. This specific clusterin proteoform seems to be a promising biomarker for myelodysplastic syndrome progression.

Název v anglickém jazyce

Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome

Popis výsledku anglicky

Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hematological malignancies.Here, we investigated proteome changes in the exosome-rich fraction of the plasma of myelodysplastic syndrome patients and healthy donors. Exosome-rich fraction of the plasma was isolated using ExoQuick™: proteomes were compared and statistically processed, proteins were identified by nanoLC-MS/MS and verified using the ExoCarta and QuickGO databases. Mann-Whitney and Spearman analyses were used to statistically analyze the data. 2D western blot was used to monitor clusterin proteoforms. Statistical analyses of the data highlighted clusterin alterations as the most significant. 2D western blot showed that the clusterin changes were caused by posttranslational modifications. Moreover, there was a notable increase in the clusterin proteoform in the exosome-rich fraction of plasma of patients with more severe myelodysplastic syndrome, this corresponded with a simultaneous decrease in their plasma. This specific clusterin proteoform seems to be a promising biomarker for myelodysplastic syndrome progression.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/GA20-10845S" target="_blank" >GA20-10845S: Individuální variabilita a patofyziologie krevní plasmy a jejich vliv na interakci s umělými povrchy potlačujícími nespecifické interakce</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

1932-6203

Svazek periodika

17

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

e0262484

Kód UT WoS článku

000741060700010

EID výsledku v databázi Scopus

2-s2.0-85122617681