A novel highly sensitive UHPLC-MS/MS method for monitoring vincristine in plasma and vitreous humor samples with potential application in retinoblastoma therapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00555467" target="_blank" >RIV/61389013:_____/22:00555467 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10452165

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X22001655?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X22001655?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2022.107337" target="_blank" >10.1016/j.microc.2022.107337</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel highly sensitive UHPLC-MS/MS method for monitoring vincristine in plasma and vitreous humor samples with potential application in retinoblastoma therapy

Popis výsledku v původním jazyce

Vincristine is a chemotherapeutic agent which is effective in treating various types of cancer, including retinoblastoma, the most common malignant intraocular tumor in children. There is a clinical requirement for a highly sensitive analytical method to monitor vincristine concentration during treatment. Therefore, we developed a simple, rapid, selective, and highly sensitive UHPLC-MS/MS method to determine vincristine concentration in plasma and vitreous humor samples. This method uses an isotopically labeled internal standard. High sensitivity was achieved by specific multiple reaction monitoring (MRM) transitions using a doubly charged acetonitrile adduct of vincristine as a precursor ion in positive mode. Finally, our protocol was fully validated to demonstrate its reliability in determining vincristine in plasma and vitreous humor samples. The method showed an excellent linear range from 0.1 to 50 ng mL−1, with precision ranging from 1.8 to 11.6% (RSD), and with accuracy (relative error %) within ± 11.8%. The matrix effect and extraction recovery for vincristine ranged from 93 to 103% and 98 to 101%, respectively.

Název v anglickém jazyce

A novel highly sensitive UHPLC-MS/MS method for monitoring vincristine in plasma and vitreous humor samples with potential application in retinoblastoma therapy

Popis výsledku anglicky

Vincristine is a chemotherapeutic agent which is effective in treating various types of cancer, including retinoblastoma, the most common malignant intraocular tumor in children. There is a clinical requirement for a highly sensitive analytical method to monitor vincristine concentration during treatment. Therefore, we developed a simple, rapid, selective, and highly sensitive UHPLC-MS/MS method to determine vincristine concentration in plasma and vitreous humor samples. This method uses an isotopically labeled internal standard. High sensitivity was achieved by specific multiple reaction monitoring (MRM) transitions using a doubly charged acetonitrile adduct of vincristine as a precursor ion in positive mode. Finally, our protocol was fully validated to demonstrate its reliability in determining vincristine in plasma and vitreous humor samples. The method showed an excellent linear range from 0.1 to 50 ng mL−1, with precision ranging from 1.8 to 11.6% (RSD), and with accuracy (relative error %) within ± 11.8%. The matrix effect and extraction recovery for vincristine ranged from 93 to 103% and 98 to 101%, respectively.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GJ19-18005Y" target="_blank" >GJ19-18005Y: Retenční mechanismus intaktních glykopeptidů v hydrofilní interakční kapalinové chromatografii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

178

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

107337

Kód UT WoS článku

000821546500006

EID výsledku v databázi Scopus

2-s2.0-85125525224