The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00564363" target="_blank" >RIV/61389013:_____/22:00564363 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.futuremedicine.com/doi/10.2217/nnm-2022-0081" target="_blank" >https://www.futuremedicine.com/doi/10.2217/nnm-2022-0081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2217/nnm-2022-0081" target="_blank" >10.2217/nnm-2022-0081</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells
Popis výsledku v původním jazyce
Exosomes are extracellular vesicles with the ability to encapsulate bioactive molecules, such as therapeutics. This study identified a new exosome mediated route of doxorubicin and poly(N-(2-hydroxypropyl)methacrylamide) (pHPMA)-bound doxorubicin trafficking in the tumor mass. Exosome loading was achieved via incubation of the therapeutics with an adherent human breast adenocarcinoma cell line and its derived spheroids. Exosomes were characterized using HPLC, nanoparticle tracking analysis (NTA) and western blotting. The therapeutics were successfully loaded into exosomes. Spheroids secreted significantly more exosomes than adherent cells and showed decreased viability after treatment with therapeutic-loaded exosomes, which confirmed successful transmission. To the best of our knowledge, this study provides the first evidence of pHPMA-drug conjugate secretion by extracellular vesicles.
Název v anglickém jazyce
The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells
Popis výsledku anglicky
Exosomes are extracellular vesicles with the ability to encapsulate bioactive molecules, such as therapeutics. This study identified a new exosome mediated route of doxorubicin and poly(N-(2-hydroxypropyl)methacrylamide) (pHPMA)-bound doxorubicin trafficking in the tumor mass. Exosome loading was achieved via incubation of the therapeutics with an adherent human breast adenocarcinoma cell line and its derived spheroids. Exosomes were characterized using HPLC, nanoparticle tracking analysis (NTA) and western blotting. The therapeutics were successfully loaded into exosomes. Spheroids secreted significantly more exosomes than adherent cells and showed decreased viability after treatment with therapeutic-loaded exosomes, which confirmed successful transmission. To the best of our knowledge, this study provides the first evidence of pHPMA-drug conjugate secretion by extracellular vesicles.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
<a href="/cs/project/LTAUSA18083" target="_blank" >LTAUSA18083: Pokročilá polymerní nanoteranostika založená na biodegradovatelných biokompatibilních nosičích</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Nanomedicine
ISSN
1743-5889
e-ISSN
1748-6963
Svazek periodika
17
Číslo periodika v rámci svazku
19
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
16
Strana od-do
1307-1322
Kód UT WoS článku
000869390900001
EID výsledku v databázi Scopus
2-s2.0-85142403255