Surface effect of iron oxide nanoparticles on the suppression of oxidative burst in cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00569400" target="_blank" >RIV/61389013:_____/23:00569400 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/23:00569400

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10876-022-02222-9" target="_blank" >https://link.springer.com/article/10.1007/s10876-022-02222-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10876-022-02222-9" target="_blank" >10.1007/s10876-022-02222-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface effect of iron oxide nanoparticles on the suppression of oxidative burst in cells

Popis výsledku v původním jazyce

Overproduction of reactive oxygen species (ROS) is an unwanted phenomenon, leading to cellular damages. The aim of this study was to investigate the ability of neat and surface-modified iron oxide nanoparticles (IONs) to eliminate ROS produced by immune cells. The employed coating included heparin (ION@Hep) or heparin and chitosan grafted with phenolic compounds famous for antioxidant properties, i.e., gallic acid (ION@Ch-G) or phloroglucinol (ION@CH-P). A total peroxyl radical-trapping potential assay showed that both types of the phenolic compounds-modified IONs exhibited superior radical scavenging activity over the neat and ION@Hep particles at 100 μg/mL. Up to ~ 75 μg/mL, the particles were non-toxic towards RAW 264.7 macrophages. Capability of the particles to limit ROS production was investigated in vitro on polymorphonuclear (PMN) cells isolated from human whole blood and expressed as an ability to reduce the oxidative burst in the stimulated cells, as well as a potential to increase the viability of bacteria cultivated with the PMN cells. The highest viability of bacteria was observed for the neat and ION@Ch-G, while the ION@Ch-G particles also the most effectively inhibited the oxidative burst. The results indicated that ROS scavenging depend on the presence of polymer and selection of phenols, enriching the IONs.

Název v anglickém jazyce

Surface effect of iron oxide nanoparticles on the suppression of oxidative burst in cells

Popis výsledku anglicky

Overproduction of reactive oxygen species (ROS) is an unwanted phenomenon, leading to cellular damages. The aim of this study was to investigate the ability of neat and surface-modified iron oxide nanoparticles (IONs) to eliminate ROS produced by immune cells. The employed coating included heparin (ION@Hep) or heparin and chitosan grafted with phenolic compounds famous for antioxidant properties, i.e., gallic acid (ION@Ch-G) or phloroglucinol (ION@CH-P). A total peroxyl radical-trapping potential assay showed that both types of the phenolic compounds-modified IONs exhibited superior radical scavenging activity over the neat and ION@Hep particles at 100 μg/mL. Up to ~ 75 μg/mL, the particles were non-toxic towards RAW 264.7 macrophages. Capability of the particles to limit ROS production was investigated in vitro on polymorphonuclear (PMN) cells isolated from human whole blood and expressed as an ability to reduce the oxidative burst in the stimulated cells, as well as a potential to increase the viability of bacteria cultivated with the PMN cells. The highest viability of bacteria was observed for the neat and ION@Ch-G, while the ION@Ch-G particles also the most effectively inhibited the oxidative burst. The results indicated that ROS scavenging depend on the presence of polymer and selection of phenols, enriching the IONs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

<a href="/cs/project/GC20-02177J" target="_blank" >GC20-02177J: Antioxidační magnetické nanočástice modifikované fenolickými sloučeninami pro léčení nemocí spojených s oxidačním stresem: Studie nano-biorozhraní</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cluster Science

ISSN

1040-7278

e-ISSN

1572-8862

Svazek periodika

34

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

323-334

Kód UT WoS článku

000746347000002

EID výsledku v databázi Scopus

2-s2.0-85123491079