Polyelectrolyte nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide-block-poly(N-(3-aminopropyl)methacrylamide] copolymers for delivery of heparin-binding proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00570910" target="_blank" >RIV/61389013:_____/23:00570910 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13440/23:43897690

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0014305723001593?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305723001593?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2023.111976" target="_blank" >10.1016/j.eurpolymj.2023.111976</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polyelectrolyte nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide-block-poly(N-(3-aminopropyl)methacrylamide] copolymers for delivery of heparin-binding proteins

Popis výsledku v původním jazyce

In addition to the delivery of therapeutic nucleic acids and low-molecular weight drugs, polyelectrolyte nanoparticles (NPs) have recently been studied as carriers for protein delivery. However, the stability of NPs during isolation steps, which ensures their easy redispersion, needs to be solved empirically for individual systems using cryoprotectants and stabilizers. To avoid the use of additives, we studied the formation of polyelectrolyte NPs consisting of a newly synthesized polycationic diblock copolymer based on poly(N-(2-hydroxypropyl)methacrylamide)-block-poly(N-(3-aminopropyl)methacrylamide) (p(HPMA-b-APMA) and heparin (Hep). The p(APMA) blocks electrostatically complexed with Hep, and the p(HPMA) blocks formed a neutral corona of NPs, limiting NP aggregation. Self-assembly was monitored through the changes in size and zeta potential of the formed NPs, which depended on the copolymer composition and the concentration of polyelectrolyte solutions. The interactions between the NP components were analysed by FTIR spectroscopy, and XPS analysis indicated the presence of p(HPMA) blocks on the surface of the NPs. The encapsulation of the model chemokine CXCL12 and basic fibroblast growth factor (FGF-2) was driven by their specific bioaffinity for Hep, resulting in a high 90 % entrapment efficiency and increased protein stability. CXCL12 was released over 48 h, while FGF-2 exhibited a sustained release of up to 38 % over four weeks. In addition, the released CXCL12 effectively stimulated the migration of macrophages and T-lymphocyte cells, indicating the preserved protein bioactivity. Considering the proven noncytotoxic performance of the NPs towards fibroblasts and mesenchymal stem cells, the polyelectrolyte NPs of p(HPMA-b-APMA) and Hep loaded with heparin-binding proteins can be considered as promising candidates for the controlled delivery of bioactive proteins in biomedical applications.

Název v anglickém jazyce

Polyelectrolyte nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide-block-poly(N-(3-aminopropyl)methacrylamide] copolymers for delivery of heparin-binding proteins

Popis výsledku anglicky

In addition to the delivery of therapeutic nucleic acids and low-molecular weight drugs, polyelectrolyte nanoparticles (NPs) have recently been studied as carriers for protein delivery. However, the stability of NPs during isolation steps, which ensures their easy redispersion, needs to be solved empirically for individual systems using cryoprotectants and stabilizers. To avoid the use of additives, we studied the formation of polyelectrolyte NPs consisting of a newly synthesized polycationic diblock copolymer based on poly(N-(2-hydroxypropyl)methacrylamide)-block-poly(N-(3-aminopropyl)methacrylamide) (p(HPMA-b-APMA) and heparin (Hep). The p(APMA) blocks electrostatically complexed with Hep, and the p(HPMA) blocks formed a neutral corona of NPs, limiting NP aggregation. Self-assembly was monitored through the changes in size and zeta potential of the formed NPs, which depended on the copolymer composition and the concentration of polyelectrolyte solutions. The interactions between the NP components were analysed by FTIR spectroscopy, and XPS analysis indicated the presence of p(HPMA) blocks on the surface of the NPs. The encapsulation of the model chemokine CXCL12 and basic fibroblast growth factor (FGF-2) was driven by their specific bioaffinity for Hep, resulting in a high 90 % entrapment efficiency and increased protein stability. CXCL12 was released over 48 h, while FGF-2 exhibited a sustained release of up to 38 % over four weeks. In addition, the released CXCL12 effectively stimulated the migration of macrophages and T-lymphocyte cells, indicating the preserved protein bioactivity. Considering the proven noncytotoxic performance of the NPs towards fibroblasts and mesenchymal stem cells, the polyelectrolyte NPs of p(HPMA-b-APMA) and Hep loaded with heparin-binding proteins can be considered as promising candidates for the controlled delivery of bioactive proteins in biomedical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

1873-1945

Svazek periodika

191

Číslo periodika v rámci svazku

13 June

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

111976

Kód UT WoS článku

000979460600001

EID výsledku v databázi Scopus

2-s2.0-85151482989