Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00574199" target="_blank" >RIV/61389013:_____/23:00574199 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13440/23:43897726

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/app.54331" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/app.54331</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/app.54331" target="_blank" >10.1002/app.54331</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging

Popis výsledku v původním jazyce

Here, we report a novel dispersion polymerization for the preparation of cross-linked poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA)-based nanogels in water/2-methoxyethanol mixture (H2O/MetCel), initiated with potassium persulfate (KPS), and stabilized with poly(vinyl alcohol) 25/140 (PVA) and sodium dodecyl sulfate (SDS). Obtained nanogels were characterized using transmission (TEM) and cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), asymmetric flow field-flow fractionation (A4F), nuclear magnetic resonance spectroscopy (NMR), and Raman spectroscopy methods in terms of size, particle size distribution, morphology, and structure. N-(2-hydroxypropyl) methacrylamide (HPMA) was copolymerized with 20 wt% ethylene dimethacrylate (EDMA) resulting in 138 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate] (PHPMA-EDMA) nanogel dispersion with irregular shape and core-shell type structure. Next, we copolymerized HPMA with 20 wt% EDMA and 10 wt% propargyl methacrylate (PMA) to incorporate reactive functionality into the final core-shell type 120 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate-co-propargyl methacrylate] (PHPMA-EDMA-PMA) nanogel dispersion. Then, the biocompatibility of PHPMA-EDMA-PMA nanogel was proved using rat mesenchymal stem cells (rMSC), and human foreskin fibroblasts (BJ). PHPMA-EDMA-PMA nanogel was fluorescently labeled with sulfo-cyanine3 azide resulting in 131 nm nanogel. We performed in vitro uptake studies with fluorescently labeled PHPMA-EDMA-PMA nanogel using rMSC showing that the fluorescently labeled PHPMA-EDMA-PMA nanogel was well-distributed in the cytosol and taken up into lysosomes.n

Název v anglickém jazyce

Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging

Popis výsledku anglicky

Here, we report a novel dispersion polymerization for the preparation of cross-linked poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA)-based nanogels in water/2-methoxyethanol mixture (H2O/MetCel), initiated with potassium persulfate (KPS), and stabilized with poly(vinyl alcohol) 25/140 (PVA) and sodium dodecyl sulfate (SDS). Obtained nanogels were characterized using transmission (TEM) and cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), asymmetric flow field-flow fractionation (A4F), nuclear magnetic resonance spectroscopy (NMR), and Raman spectroscopy methods in terms of size, particle size distribution, morphology, and structure. N-(2-hydroxypropyl) methacrylamide (HPMA) was copolymerized with 20 wt% ethylene dimethacrylate (EDMA) resulting in 138 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate] (PHPMA-EDMA) nanogel dispersion with irregular shape and core-shell type structure. Next, we copolymerized HPMA with 20 wt% EDMA and 10 wt% propargyl methacrylate (PMA) to incorporate reactive functionality into the final core-shell type 120 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate-co-propargyl methacrylate] (PHPMA-EDMA-PMA) nanogel dispersion. Then, the biocompatibility of PHPMA-EDMA-PMA nanogel was proved using rat mesenchymal stem cells (rMSC), and human foreskin fibroblasts (BJ). PHPMA-EDMA-PMA nanogel was fluorescently labeled with sulfo-cyanine3 azide resulting in 131 nm nanogel. We performed in vitro uptake studies with fluorescently labeled PHPMA-EDMA-PMA nanogel using rMSC showing that the fluorescently labeled PHPMA-EDMA-PMA nanogel was well-distributed in the cytosol and taken up into lysosomes.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Polymer Science

ISSN

0021-8995

e-ISSN

1097-4628

Svazek periodika

140

Číslo periodika v rámci svazku

34

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

e54331

Kód UT WoS článku

001024203900001

EID výsledku v databázi Scopus

2-s2.0-85164310907