Liquid chromatography methods as a solution to inaccuracies associated with purity assessment of citric acid-based carbon dots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00588313" target="_blank" >RIV/61389013:_____/24:00588313 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X24013523?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X24013523?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.111240" target="_blank" >10.1016/j.microc.2024.111240</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Liquid chromatography methods as a solution to inaccuracies associated with purity assessment of citric acid-based carbon dots

Popis výsledku v původním jazyce

Herein, several analytical techniques aimed on validation of separation effectiveness of free molecular fluorophores from citric acid-based carbon dots were elaborated and compared. Generally, chromatographic methods were found more reliable than steady-state optical measurements, pointing at the LC-FLD (liquid chromatography coupled to fluorescence detector) protocols as the most sensitive. Furthermore, the applicability of the frequently reported 1H NMR technique for confirmation of the absence of highly fluorescent free low-molecular weight species in carbon dots samples was called into question. It was found that, in the case of effectively PL emitting compounds (such as 5-oxo-2,3- dihydro-5H-[1,3]thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid (TPA)), the lowest concentration that could be detected with above technique (0.08 mg mL−1 of TPA) is higher than the concentration at which the particular fluorophore shows the brightest PL emission (∼0.01 mg mL−1 of TPA).

Název v anglickém jazyce

Liquid chromatography methods as a solution to inaccuracies associated with purity assessment of citric acid-based carbon dots

Popis výsledku anglicky

Herein, several analytical techniques aimed on validation of separation effectiveness of free molecular fluorophores from citric acid-based carbon dots were elaborated and compared. Generally, chromatographic methods were found more reliable than steady-state optical measurements, pointing at the LC-FLD (liquid chromatography coupled to fluorescence detector) protocols as the most sensitive. Furthermore, the applicability of the frequently reported 1H NMR technique for confirmation of the absence of highly fluorescent free low-molecular weight species in carbon dots samples was called into question. It was found that, in the case of effectively PL emitting compounds (such as 5-oxo-2,3- dihydro-5H-[1,3]thiazolo[3,2-a]pyridine-3,7-dicarboxylic acid (TPA)), the lowest concentration that could be detected with above technique (0.08 mg mL−1 of TPA) is higher than the concentration at which the particular fluorophore shows the brightest PL emission (∼0.01 mg mL−1 of TPA).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

205

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

111240

Kód UT WoS článku

001280603400001

EID výsledku v databázi Scopus

2-s2.0-85199256130