Individual and simultaneous encapsulation and delivery of incompatible dyes in biocompatible multicompartment terpolymer micelles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00588484" target="_blank" >RIV/61389013:_____/24:00588484 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10483859

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0014305724006050?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305724006050?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.eurpolymj.2024.113344" target="_blank" >10.1016/j.eurpolymj.2024.113344</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Individual and simultaneous encapsulation and delivery of incompatible dyes in biocompatible multicompartment terpolymer micelles

Popis výsledku v původním jazyce

Multicompartment micelles (MCMs) can deliver incompatible drug payloads because MCMs derive from the self-assembly of a terpolymer containing one hydrophilic block and two incompatible solvophobic blocks. In particular, MCMs resulting from the self-assembly of poly((sulfamate-carboxylate)isoprene)-block-polystyrene-block-poly(ethylene oxide) (PISC-b-PS-b-PEO) undergo irreversible transition to regular micelles at an alkaline pH. However, several aspects of these MCMs remain unexplored, such as the chemical transformation of PISC-b-PS-b-PEO into an amino-acid functional terpolymer, potential applications of these nanoparticles as dual drug delivery systems and their biocompatibility. In exploring these opportunities, we found that more than 90% of N-S bonds of sulfamate groups (of the PISC block) are cleaved upon acidic treatment of PISC-b-PS-b-PEO terpolymers, which are chemically transformed into a new polyzwitterion triblock terpolymer, that is, poly((amino-carboxylate)isoprene)-block-polystyrene-block-poly(ethylene oxide) (PACIS-b-PS-b-PEO), but retain their MCM morphology. Into these MCMs, two incompatible (Nile Red (hydrophobic) and eosin (hydrophilic)) guest molecules can be loaded separately and together in the predesigned cores without significantly disturbing the structure, as shown by cryo-TEM and light scattering. In addition to their high stability, PACIS-b-PS-b-PEO MCMs are also pH-responsive, whether loaded or unloaded, based on fluorescence spectroscopy and light scattering. In cytotoxicity tests, loaded and unloaded PACIS-b-PS-b-PEO nanoparticles proved nontoxic to various cell lines, including Balb/3T3 and MCF10A, with efficient cellular uptake, as shown by fluorescence imaging. Therefore, our findings highlight PACIS-b-PS-b-PEO as a versatile platform for preparing MCMs for multiple drug delivery.

Název v anglickém jazyce

Individual and simultaneous encapsulation and delivery of incompatible dyes in biocompatible multicompartment terpolymer micelles

Popis výsledku anglicky

Multicompartment micelles (MCMs) can deliver incompatible drug payloads because MCMs derive from the self-assembly of a terpolymer containing one hydrophilic block and two incompatible solvophobic blocks. In particular, MCMs resulting from the self-assembly of poly((sulfamate-carboxylate)isoprene)-block-polystyrene-block-poly(ethylene oxide) (PISC-b-PS-b-PEO) undergo irreversible transition to regular micelles at an alkaline pH. However, several aspects of these MCMs remain unexplored, such as the chemical transformation of PISC-b-PS-b-PEO into an amino-acid functional terpolymer, potential applications of these nanoparticles as dual drug delivery systems and their biocompatibility. In exploring these opportunities, we found that more than 90% of N-S bonds of sulfamate groups (of the PISC block) are cleaved upon acidic treatment of PISC-b-PS-b-PEO terpolymers, which are chemically transformed into a new polyzwitterion triblock terpolymer, that is, poly((amino-carboxylate)isoprene)-block-polystyrene-block-poly(ethylene oxide) (PACIS-b-PS-b-PEO), but retain their MCM morphology. Into these MCMs, two incompatible (Nile Red (hydrophobic) and eosin (hydrophilic)) guest molecules can be loaded separately and together in the predesigned cores without significantly disturbing the structure, as shown by cryo-TEM and light scattering. In addition to their high stability, PACIS-b-PS-b-PEO MCMs are also pH-responsive, whether loaded or unloaded, based on fluorescence spectroscopy and light scattering. In cytotoxicity tests, loaded and unloaded PACIS-b-PS-b-PEO nanoparticles proved nontoxic to various cell lines, including Balb/3T3 and MCF10A, with efficient cellular uptake, as shown by fluorescence imaging. Therefore, our findings highlight PACIS-b-PS-b-PEO as a versatile platform for preparing MCMs for multiple drug delivery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Polymer Journal

ISSN

0014-3057

e-ISSN

1873-1945

Svazek periodika

218

Číslo periodika v rámci svazku

18 September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

113344

Kód UT WoS článku

001286786000001

EID výsledku v databázi Scopus

2-s2.0-85199955102