Bio-responsive polymers for dual 31P/19F-magnetic resonance to detect reactive oxygen species in vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00598010" target="_blank" >RIV/61389013:_____/24:00598010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/24:00085028 RIV/00216208:11110/24:10483260

Výsledek na webu

<a href="https://www.nature.com/articles/s43246-024-00623-z" target="_blank" >https://www.nature.com/articles/s43246-024-00623-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s43246-024-00623-z" target="_blank" >10.1038/s43246-024-00623-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bio-responsive polymers for dual 31P/19F-magnetic resonance to detect reactive oxygen species in vivo

Popis výsledku v původním jazyce

Biocompatible metal-free agents are emerging as a promising alternative to commercial magnetic resonance (MR) contrast agents, but there is an additional need for novel probes with enhanced responsiveness in preclinical MR testing to effectively target diverse pathological conditions. To address this, we develop hydrophilic phospho-/fluoropolymers as dual MR probes. Incorporating thiophosphoester groups (P = S) into the polymer structure produces a distinct chemical shift (~59 ppm) in phosphorus MR (31P-MR), reducing biological signals interference. Reactive oxygen species (ROS) oxidize the P = S groups, causing a detectable shift in 31P-MR, enabling precise localization of ROS, abundant in inflammation and cancer. To enhance this capability, bioinert trifluoromethyl groups (CF3) are added, creating a “hotspot” for fluorine MR (19F-MR), aiding in vivo localization. Both in vitro and in vivo testing demonstrate the probe’s high specificity and responsiveness, underscoring its potential as a sensitive ROS sensor and dual MR-traceable tool in cancer research.

Název v anglickém jazyce

Bio-responsive polymers for dual 31P/19F-magnetic resonance to detect reactive oxygen species in vivo

Popis výsledku anglicky

Biocompatible metal-free agents are emerging as a promising alternative to commercial magnetic resonance (MR) contrast agents, but there is an additional need for novel probes with enhanced responsiveness in preclinical MR testing to effectively target diverse pathological conditions. To address this, we develop hydrophilic phospho-/fluoropolymers as dual MR probes. Incorporating thiophosphoester groups (P = S) into the polymer structure produces a distinct chemical shift (~59 ppm) in phosphorus MR (31P-MR), reducing biological signals interference. Reactive oxygen species (ROS) oxidize the P = S groups, causing a detectable shift in 31P-MR, enabling precise localization of ROS, abundant in inflammation and cancer. To enhance this capability, bioinert trifluoromethyl groups (CF3) are added, creating a “hotspot” for fluorine MR (19F-MR), aiding in vivo localization. Both in vitro and in vivo testing demonstrate the probe’s high specificity and responsiveness, underscoring its potential as a sensitive ROS sensor and dual MR-traceable tool in cancer research.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Communications Materials

ISSN

2662-4443

e-ISSN

2662-4443

Svazek periodika

5

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

178

Kód UT WoS článku

001307836000001

EID výsledku v databázi Scopus

2-s2.0-85203260497