Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F14%3A00436700" target="_blank" >RIV/61389021:_____/14:00436700 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV)

Popis výsledku v původním jazyce

Předneseno na:HAKONEXIV,Greifswald,Inactivation of Escherichia coli using non-thermal surface dielectric barrier discharges (SDBD?s) was widely studied; however, the complete mechanism of plasma-assisted inactivation is still not known.We investigated processes involved in the plasma-assisted inactivation with a particular emphasis on how microorganisms response to plasma-induced stress.The single streamer filament SDBD was produced in coplanar electrode geometry and driven by amplitude-modulated AC high voltage waveforms in humid synthetic air at atmospheric pressure.The response to plasma-induced stress was estimated by conventional cultivation,sub-lethal injury assay, resazurin assay and LIVE/DEAD BacLight BacterialViability kit. Treated bacteria partly lost ability to grow precisely and became injured and cultivable or even morphologically intact(viable/active) but non-culturable (VBNC/ABNC).Formation of damaged membranes was observed through propidium iodide,which labels only

Název v anglickém jazyce

Inactivation of Escherichia coli by surface streamer discharges(HAKONEXIV)

Popis výsledku anglicky

Předneseno na:HAKONEXIV,Greifswald,Inactivation of Escherichia coli using non-thermal surface dielectric barrier discharges (SDBD?s) was widely studied; however, the complete mechanism of plasma-assisted inactivation is still not known.We investigated processes involved in the plasma-assisted inactivation with a particular emphasis on how microorganisms response to plasma-induced stress.The single streamer filament SDBD was produced in coplanar electrode geometry and driven by amplitude-modulated AC high voltage waveforms in humid synthetic air at atmospheric pressure.The response to plasma-induced stress was estimated by conventional cultivation,sub-lethal injury assay, resazurin assay and LIVE/DEAD BacLight BacterialViability kit. Treated bacteria partly lost ability to grow precisely and became injured and cultivable or even morphologically intact(viable/active) but non-culturable (VBNC/ABNC).Formation of damaged membranes was observed through propidium iodide,which labels only

Druh

O - Ostatní výsledky

CEP obor

BL - Fyzika plasmatu a výboje v plynech

OECD FORD obor

—

Projekt

<a href="/cs/project/LD13010" target="_blank" >LD13010: Nové zdroje atmosférického plazmatu na bázi povrchových bariérových výbojů pro biomedicínské aplikace.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů