In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F15%3A00441045" target="_blank" >RIV/61389021:_____/15:00441045 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/15:00441045 RIV/00216208:11110/15:10286360

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.019" target="_blank" >http://dx.doi.org/10.1016/j.bioelechem.2014.08.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.019" target="_blank" >10.1016/j.bioelechem.2014.08.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques

Popis výsledku v původním jazyce

Shock waves can cause significant cytotoxic effects in tumor cells and tissues both in vitro and in vivo.However,understanding the mechanisms of shock wave interaction with tissues is limited.We have studied in vivo effects of focused shock waves induced in the syngeneic sarcoma tumor model using the TUNEL assay,immunohistochemical detection of caspase-3 and hematoxylin-eosin staining.Shock waves were produced by a multichannel pulsed-electrohydraulic discharge generator with a cylindrical ceramic-coated electrode.In tumors treated with shock waves,a large area of damaged tissue was detected which was clearly differentiated from intact tissue. Localization and a cone-shaped region of tissue damage visualized by TUNEL reaction apparently correlated with the conical shape and direction of shock wave propagation determined by high-speed shadowgraphy.A strong TUNEL reaction of nuclei and nucleus fragments in tissue exposed to shock waves suggested apoptosis in this destroyed tumor area.

Název v anglickém jazyce

In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques

Popis výsledku anglicky

Shock waves can cause significant cytotoxic effects in tumor cells and tissues both in vitro and in vivo.However,understanding the mechanisms of shock wave interaction with tissues is limited.We have studied in vivo effects of focused shock waves induced in the syngeneic sarcoma tumor model using the TUNEL assay,immunohistochemical detection of caspase-3 and hematoxylin-eosin staining.Shock waves were produced by a multichannel pulsed-electrohydraulic discharge generator with a cylindrical ceramic-coated electrode.In tumors treated with shock waves,a large area of damaged tissue was detected which was clearly differentiated from intact tissue. Localization and a cone-shaped region of tissue damage visualized by TUNEL reaction apparently correlated with the conical shape and direction of shock wave propagation determined by high-speed shadowgraphy.A strong TUNEL reaction of nuclei and nucleus fragments in tissue exposed to shock waves suggested apoptosis in this destroyed tumor area.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

103-110

Kód UT WoS článku

000351977000017

EID výsledku v databázi Scopus

2-s2.0-84923383214