Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F15%3A00444287" target="_blank" >RIV/61389021:_____/15:00444287 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.018" target="_blank" >http://dx.doi.org/10.1016/j.bioelechem.2014.08.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.018" target="_blank" >10.1016/j.bioelechem.2014.08.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Popis výsledku v původním jazyce

Electrical discharge plasmas can efficiently inactivate various microorganisms. Inactivation mechanisms caused by plasma, however, are not fully understood because of the complexity of both the plasma and biological systems.We investigated plasma-inducedinactivation of Escherichia coli in water and mechanisms by which plasma affects bacterial cell membrane integrity.Atmospheric pressure argon plasma jet generated at ambient air in direct contact with bacterial suspension was used as a plasma source.Wedetermined significantly lower counts of E. coli after treatment by plasma when they were assayed using a conventional cultivation technique than using a fluorescence-based LIVE/DEAD staining method,which indicated that bacteria may have entered the viable-but-nonculturable state (VBNC).We did not achieve resuscitation of these non-culturable cells, however,we detected their metabolic activity through the analysis of cellular mRNA,which suggests that cells may have been rather in the

Název v anglickém jazyce

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Popis výsledku anglicky

Electrical discharge plasmas can efficiently inactivate various microorganisms. Inactivation mechanisms caused by plasma, however, are not fully understood because of the complexity of both the plasma and biological systems.We investigated plasma-inducedinactivation of Escherichia coli in water and mechanisms by which plasma affects bacterial cell membrane integrity.Atmospheric pressure argon plasma jet generated at ambient air in direct contact with bacterial suspension was used as a plasma source.Wedetermined significantly lower counts of E. coli after treatment by plasma when they were assayed using a conventional cultivation technique than using a fluorescence-based LIVE/DEAD staining method,which indicated that bacteria may have entered the viable-but-nonculturable state (VBNC).We did not achieve resuscitation of these non-culturable cells, however,we detected their metabolic activity through the analysis of cellular mRNA,which suggests that cells may have been rather in the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LD14080" target="_blank" >LD14080: Chemické procesy vyvolané výbojovým plazmatem ve vodných roztocích a jejich využití pro nové aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

7-14

Kód UT WoS článku

000351977000003

EID výsledku v databázi Scopus

2-s2.0-84923363431