DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023001" target="_blank" >RIV/61389030:_____/02:56023001 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.

Popis výsledku v původním jazyce

The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average ( S.E.) median tailmoment value was only 4.85 1.00 |xm in nuclei isolated from 2-day-old cells compared to 72.33 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC^, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymesand macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth. ř 2002 Elsevier Science B.V. All rights reserved.

Název v anglickém jazyce

DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.

Popis výsledku anglicky

The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average ( S.E.) median tailmoment value was only 4.85 1.00 |xm in nuclei isolated from 2-day-old cells compared to 72.33 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC^, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymesand macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth. ř 2002 Elsevier Science B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F02%2F0400" target="_blank" >GA521/02/0400: Genotoxicita mutagenů zevního prostředí detekovaná u vyšších rostlin pomocí molekulárně-biologických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mutation Research

ISSN

1383-5718

e-ISSN

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Svazek periodika

514

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

147-152

Kód UT WoS článku

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EID výsledku v databázi Scopus

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