Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023057" target="_blank" >RIV/61389030:_____/02:56023057 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.

Popis výsledku v původním jazyce

Phosphatidylcholine, labeled by two fluorescent fatty acids, was fed to cultured plant cells (Petrosilenum crispum, L.; VBI-0, Nicotiana benthiana, L.) and fluorescent diacylglycerol (DAG) was the major metabolite. When a glycoprotein elicitor, derived from Phytophthora sojae, was applied to the parsley cells and the small protein cryptogein from Phytophthora cryptogea was applied to the tobacco cells. these signal substances strongly and rapidly decreased the pool of fluorescent diacylglycerol and weakly increased the pool of free fluorescent fatty acid and of fluorescent lysophosphatidylcholine. The cells responded in a very similar way to the application of mastoparan, a wasp venom peptide. As phosphatidic acid was only a very minor fluorescent metabolite DAG is hypothesized to arise by the action of a phosphatidylcholine-hydrolyzing phospholipase C which was down-regulated by elicitors. Up-regulation of a phospholipase A by elicitors is also suggested by these results. This is the

Název v anglickém jazyce

Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.

Popis výsledku anglicky

Phosphatidylcholine, labeled by two fluorescent fatty acids, was fed to cultured plant cells (Petrosilenum crispum, L.; VBI-0, Nicotiana benthiana, L.) and fluorescent diacylglycerol (DAG) was the major metabolite. When a glycoprotein elicitor, derived from Phytophthora sojae, was applied to the parsley cells and the small protein cryptogein from Phytophthora cryptogea was applied to the tobacco cells. these signal substances strongly and rapidly decreased the pool of fluorescent diacylglycerol and weakly increased the pool of free fluorescent fatty acid and of fluorescent lysophosphatidylcholine. The cells responded in a very similar way to the application of mastoparan, a wasp venom peptide. As phosphatidic acid was only a very minor fluorescent metabolite DAG is hypothesized to arise by the action of a phosphatidylcholine-hydrolyzing phospholipase C which was down-regulated by elicitors. Up-regulation of a phospholipase A by elicitors is also suggested by these results. This is the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F00%2F1332" target="_blank" >GA522/00/1332: Úloha fosfolipas v mechanismu přenosu signálů u rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ISSN

0006-291X

e-ISSN

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Svazek periodika

293

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

766-770

Kód UT WoS článku

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EID výsledku v databázi Scopus

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