Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033044" target="_blank" >RIV/61389030:_____/03:56033044 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/03:56033044 RIV/61388963:_____/03:56033044

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

Popis výsledku v původním jazyce

The growth and viability of tobacco cells (Nicotiana tabacum L.) immobilized in alginate or pectate were' monitored during their cultivation by using two-dimensional fluorescence spectroscopy (2-D FS). The cell growth was followed via the fluorescence ofamino acids in proteins. The correlation between the tryptophan fluorescence and the cell biomass inside the alginate beads was verified by comparison with the dry weight of the cells. The determination of biomass content or cell viability by measurement of the intensity of NAD(P)H fluorescence was found unsuitable. Cell viability was estimated by determination of cell esterase activity using fluorescein diacetate as a fluorogenic substrate. The fluorescence intensities of both fluorophores, tryptophanand fluorescein, were determined by scanning a 2-D FS spectrum of intact beads in front face cuvette. Using this technique the effect of organic solvent, dimethyl sulfoxide, on the growth and metabolic activities of cells within the bead

Název v anglickém jazyce

Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

Popis výsledku anglicky

The growth and viability of tobacco cells (Nicotiana tabacum L.) immobilized in alginate or pectate were' monitored during their cultivation by using two-dimensional fluorescence spectroscopy (2-D FS). The cell growth was followed via the fluorescence ofamino acids in proteins. The correlation between the tryptophan fluorescence and the cell biomass inside the alginate beads was verified by comparison with the dry weight of the cells. The determination of biomass content or cell viability by measurement of the intensity of NAD(P)H fluorescence was found unsuitable. Cell viability was estimated by determination of cell esterase activity using fluorescein diacetate as a fluorogenic substrate. The fluorescence intensities of both fluorophores, tryptophanand fluorescein, were determined by scanning a 2-D FS spectrum of intact beads in front face cuvette. Using this technique the effect of organic solvent, dimethyl sulfoxide, on the growth and metabolic activities of cells within the bead

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical industry

ISSN

0354-7531

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

HR - Chorvatská republika

Počet stran výsledku

4

Strana od-do

632-635

Kód UT WoS článku

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EID výsledku v databázi Scopus

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