Inhibition of Cytokinin-Glucose Conjugation in Derooted Radish, Tobacco and Bean Seedlings.

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of Cytokinin-Glucose Conjugation in Derooted Radish, Tobacco and Bean Seedlings.

Popis výsledku v původním jazyce

Four inhibitors of cyclin-dependent kinases, olomoucine, bohem-ine, roscovitine and isopropyl-olomoucine were tested for their ability to modify the conversion of exogenous tritiated dihydrozeatin ([3H]DHZ) in species differing in their pattern of cytokinin sequestering. In radish (Raph-anus sativus L.), species with predominant cytokinin N-glucosylation pathway, application of inhibitors (lOOuM) decreased conversion of [3H]DHZ into 7N- and 9N-glucosides. In common bean (Phaseolus vulgaris L.) cytokininO-glucosylation is the prevailing deactivation mechanism and the tested inhibitors did not influence significantly the conversion of [3H]DHZ. The down-regulation of active cytokinin levels in tobacco (Nicotiana tabacum L.) is achieved by both 7N-glucosylation and degradation by cytokinin oxid-ase/dehydrogenase (CKX). The inhibition of 7N-glucosylation in this species stimulated the conversion of [3H]DHZ to nucleotide and riboside. The tested purine analogues have been proved to be selec

Název v anglickém jazyce

Inhibition of Cytokinin-Glucose Conjugation in Derooted Radish, Tobacco and Bean Seedlings.

Popis výsledku anglicky

Four inhibitors of cyclin-dependent kinases, olomoucine, bohem-ine, roscovitine and isopropyl-olomoucine were tested for their ability to modify the conversion of exogenous tritiated dihydrozeatin ([3H]DHZ) in species differing in their pattern of cytokinin sequestering. In radish (Raph-anus sativus L.), species with predominant cytokinin N-glucosylation pathway, application of inhibitors (lOOuM) decreased conversion of [3H]DHZ into 7N- and 9N-glucosides. In common bean (Phaseolus vulgaris L.) cytokininO-glucosylation is the prevailing deactivation mechanism and the tested inhibitors did not influence significantly the conversion of [3H]DHZ. The down-regulation of active cytokinin levels in tobacco (Nicotiana tabacum L.) is achieved by both 7N-glucosylation and degradation by cytokinin oxid-ase/dehydrogenase (CKX). The inhibition of 7N-glucosylation in this species stimulated the conversion of [3H]DHZ to nucleotide and riboside. The tested purine analogues have been proved to be selec

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bulgarian Journal of Plant Physiology

ISSN

1310-4586

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

1/2

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

8

Strana od-do

13-20

Kód UT WoS článku

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EID výsledku v databázi Scopus

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