Molekulární cytogenetika a cytometrie banánovníku (Musa spp.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107482" target="_blank" >RIV/61389030:_____/04:00107482 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/04:00002310

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular cytogenetics and cytometry of banana (Musa spp.)

Popis výsledku v původním jazyce

This work focused on the development of flow cytometry and molecular cytogenetics for Musa, and on the use of new tools for detailed and more efficient characterisation and analysis of its genome at the nuclear and chromosomal levels. Using flow cytometry, genome size of Musa was determined for the first time, and the extent of its variation was assessed. The method was invaluable for the rapid estimation of ploidy levels and detection of mixoploidy. While the method remains an important research tool,it is being increasingly used in Musa breeding programmes. Molecular cytogenetical methods that permit physical mapping of DNA sequences to Musa chromosomes were developed and used first to obtain physical chromosome landmarks and to characterise variousrepetitive DNA sequences. A novel method for preparation of high molecular weight DNA has been developed, and work is under way to construct the first Musa B-genome BAG library. The results obtained represent major steps forward in ou...

Název v anglickém jazyce

Molecular cytogenetics and cytometry of banana (Musa spp.)

Popis výsledku anglicky

This work focused on the development of flow cytometry and molecular cytogenetics for Musa, and on the use of new tools for detailed and more efficient characterisation and analysis of its genome at the nuclear and chromosomal levels. Using flow cytometry, genome size of Musa was determined for the first time, and the extent of its variation was assessed. The method was invaluable for the rapid estimation of ploidy levels and detection of mixoploidy. While the method remains an important research tool,it is being increasingly used in Musa breeding programmes. Molecular cytogenetical methods that permit physical mapping of DNA sequences to Musa chromosomes were developed and used first to obtain physical chromosome landmarks and to characterise variousrepetitive DNA sequences. A novel method for preparation of high molecular weight DNA has been developed, and work is under way to construct the first Musa B-genome BAG library. The results obtained represent major steps forward in ou...

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA6038204" target="_blank" >IAA6038204: Konstrukce knihovny DNA banánovníku klonované ve vektoru BAC (Bacterial artificial chromosome) pro fyzické mapování genomu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Počet stran výsledku

15

Strana od-do

229-244

Počet stran knihy

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Název nakladatele

Science Publishers, Inc

Místo vydání

Enfield

Kód UT WoS kapitoly

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