Analýza sekvencí chromozomu 5 Pisum sativum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109137" target="_blank" >RIV/61389030:_____/04:00109137 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of nucleotide sequence of Pisum sativum chromosome-5-specific library

Popis výsledku v původním jazyce

Sequencing of clones derived from short insert chromosome-5-specific DNA library of pea may provide valuable information of chromosome structure. Our library was constructed by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification using DNA of flow-sorted chromosomes as template. Analysis of nucleotide sequence of 200 randomly selected clones indicated that about 20% of clones were overrepresented in the library. The nucleotide sequence of 155 original chromosome-specific fragments was revealed. Sequence homology analysis showed that minority of fragments were similar only to uncharacterised ge-nomic sequences of different plant species. However, the set of sequences revealed different ranges of similarity to plant genes and different types of moderate repetitive elements. The results suggested equal reflection of real genome composition in the library clones, with the exception of short tandem repeats sequences. Shotgun characterisation implied a rathe...

Název v anglickém jazyce

Analysis of nucleotide sequence of Pisum sativum chromosome-5-specific library

Popis výsledku anglicky

Sequencing of clones derived from short insert chromosome-5-specific DNA library of pea may provide valuable information of chromosome structure. Our library was constructed by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification using DNA of flow-sorted chromosomes as template. Analysis of nucleotide sequence of 200 randomly selected clones indicated that about 20% of clones were overrepresented in the library. The nucleotide sequence of 155 original chromosome-specific fragments was revealed. Sequence homology analysis showed that minority of fragments were similar only to uncharacterised ge-nomic sequences of different plant species. However, the set of sequences revealed different ranges of similarity to plant genes and different types of moderate repetitive elements. The results suggested equal reflection of real genome composition in the library clones, with the exception of short tandem repeats sequences. Shotgun characterisation implied a rathe...

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F03%2F0595" target="_blank" >GA521/03/0595: Cílená izolace molekulárních markerů a srovnávací mapování genomů cizrny, bobu a hrachu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the International Workshop 'Understanding the Plant Genomeö

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

10

Strana od-do

79-88

Název nakladatele

Institute of Plant Genetics PAS

Místo vydání

Poznan

Místo konání akce

Poznan

Datum konání akce

2. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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