Nová metoda isolace a subfrakcionace rostlinných mitochondrií vhodná pro proteomické analýzy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109145" target="_blank" >RIV/61389030:_____/04:00109145 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New method of plant mitochondria isolation and sub-fractionation for proteomic analyses

Popis výsledku v původním jazyce

A new method for the isolation and sub-fractionation of mitochondria from plant tissues is described. This method was used for the analysis of proteins isolated from a pure total mitochondrial fraction and of protein complexes obtained from two mitochondrial sub-fractions, membrane-bound and matrix, from in vitro cultivated tobacco pollen tubes. The method comprised: a new plant tissue homogenization procedure: differential centrifugation of the homogenates in a Percoll gradient; mitochondria immobilization together with Percoll removal by filtration through an uncharged nylon membrane. Mitochondria trapped on the nylon membrane were used for (1) rapid protein extraction; (2) quantitative phenol-chloroform protein extraction; (3) mitochondria sub-fractionation into membrane-bound and matrix fractions. The new method was found to be more efficient than previously published protocols, requiring the use of as little as 180 mg of fresh plant material. It is very fast, reliable, and suit...

Název v anglickém jazyce

New method of plant mitochondria isolation and sub-fractionation for proteomic analyses

Popis výsledku anglicky

A new method for the isolation and sub-fractionation of mitochondria from plant tissues is described. This method was used for the analysis of proteins isolated from a pure total mitochondrial fraction and of protein complexes obtained from two mitochondrial sub-fractions, membrane-bound and matrix, from in vitro cultivated tobacco pollen tubes. The method comprised: a new plant tissue homogenization procedure: differential centrifugation of the homogenates in a Percoll gradient; mitochondria immobilization together with Percoll removal by filtration through an uncharged nylon membrane. Mitochondria trapped on the nylon membrane were used for (1) rapid protein extraction; (2) quantitative phenol-chloroform protein extraction; (3) mitochondria sub-fractionation into membrane-bound and matrix fractions. The new method was found to be more efficient than previously published protocols, requiring the use of as little as 180 mg of fresh plant material. It is very fast, reliable, and suit...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LZ1K03018" target="_blank" >LZ1K03018: Honys: Identifikace promotorů mikrosporově specifických genů a jejich využití jako nástrojů pro molekulární biologii rostlin</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

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Svazek periodika

167

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

7

Strana od-do

389-395

Kód UT WoS článku

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EID výsledku v databázi Scopus

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