Konstrukce BAC knihovny pro studium struktury a evoluce genomu banánovníku (M. balbisiana)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109158" target="_blank" >RIV/61389030:_____/04:00109158 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/04:00002320

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome

Popis výsledku v původním jazyce

The first bacterial artificial chromosome (BAC) library of the banana species Musa balbisiana žPisang Klutuk WulungŽ (PKW BAC library) was constructed and characterized. One improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. The use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid DNA. Furthermore, the usefulness of the inducible pCC1BAC vector to obtain a higher amount of BAC DNA was demonstrated. The PKW BAC library represents nine haploid genome equivalents of M. balbisiana and its mean insert size is 135 kb. It consists of two sublibraries, of which the first one (SN sublibrarywith 24 960 clones) was prepared according to an improved standard nuclei isolation protocol, whereas the second (FN sublibrary with 11 904 clones) was obtained from flow-sorted nuclei. Screening with 12 RFLP probes, which were geneti...

Název v anglickém jazyce

Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome

Popis výsledku anglicky

The first bacterial artificial chromosome (BAC) library of the banana species Musa balbisiana žPisang Klutuk WulungŽ (PKW BAC library) was constructed and characterized. One improved and one novel protocol for nuclei isolation were employed to overcome problems caused by high levels of polyphenols and polysaccharides present in leaf tissues. The use of flow cytometry to purify cell nuclei eliminated contamination with secondary metabolites and plastid DNA. Furthermore, the usefulness of the inducible pCC1BAC vector to obtain a higher amount of BAC DNA was demonstrated. The PKW BAC library represents nine haploid genome equivalents of M. balbisiana and its mean insert size is 135 kb. It consists of two sublibraries, of which the first one (SN sublibrarywith 24 960 clones) was prepared according to an improved standard nuclei isolation protocol, whereas the second (FN sublibrary with 11 904 clones) was obtained from flow-sorted nuclei. Screening with 12 RFLP probes, which were geneti...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome

ISSN

1480-3321

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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