Stanovení stability ploidie procesu somatické embryogeneze u Quercus suber L. pomocí průtokové cytometrie

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028181" target="_blank" >RIV/61389030:_____/05:00028181 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry

Popis výsledku v původním jazyce

Flow cytometry analyses were used to verify the ploidy stability of Quercus suber L. somatic embryogenesis process. Leaf explants of cork oak trees were inoculated on MS medium with 2,4-D and zeatin. Calluses with embryogenic structures were transferredto fresh MS medium without growth regulators and somatic embryo evolution was followed. Flow cytometry was employed to estimate DNA ploidy levels and nuclear DNA content of somatic embryos and leaves from mother plants. No significant differences (P?? 0.05) were detected among embry-os, and between the embryos and the mother plants. Also, after conversion of these embryos, no significant morphological differences were observed among the somatic embryoderived plants. These results indicate that embryo cultures and converted plantlets were stable with regard to ploidy level.

Název v anglickém jazyce

Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry

Popis výsledku anglicky

Flow cytometry analyses were used to verify the ploidy stability of Quercus suber L. somatic embryogenesis process. Leaf explants of cork oak trees were inoculated on MS medium with 2,4-D and zeatin. Calluses with embryogenic structures were transferredto fresh MS medium without growth regulators and somatic embryo evolution was followed. Flow cytometry was employed to estimate DNA ploidy levels and nuclear DNA content of somatic embryos and leaves from mother plants. No significant differences (P?? 0.05) were detected among embry-os, and between the embryos and the mother plants. Also, after conversion of these embryos, no significant morphological differences were observed among the somatic embryoderived plants. These results indicate that embryo cultures and converted plantlets were stable with regard to ploidy level.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Planta

ISSN

0032-0935

e-ISSN

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Svazek periodika

221

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

815-822

Kód UT WoS článku

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EID výsledku v databázi Scopus

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