Isolace a charakterizace parakrystalických struktur z transgenního Pssu-ipt tabáku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00030607" target="_blank" >RIV/61389030:_____/05:00030607 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/05:00008748

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco

Popis výsledku v původním jazyce

Distinct crystalloids were found in chloroplasts of transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) overproducing endogenous cytokinins. They were present both in rooted (T) and grafted (TC) transgenic plants contrary to control tobacco (C). The fractions enriched by crystalloids were isolated from chloroplasts using a continuous or a discontinuous Percoll gradient. The results obtained from chlorophyll (Chl) fluorescence emission spectra at 77 K indicated the presence of aggregates of light-harvesting complex proteins (LHC II) that was not connected to reaction centres of Photosystem II both in isolated chloroplasts and in the fraction of 80% Percoll gradient from both types of transgenic tobacco. Further analyses, i.e. pigmentcontents, polypeptide composition by SDS-PAGE, and immunoblotting support our hypothesis that crystalloids inside chloroplasts of transgenic tobacco are formed by LHC II aggregates. Treatment with two distinct detergents, chosen with ...

Název v anglickém jazyce

Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco

Popis výsledku anglicky

Distinct crystalloids were found in chloroplasts of transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) overproducing endogenous cytokinins. They were present both in rooted (T) and grafted (TC) transgenic plants contrary to control tobacco (C). The fractions enriched by crystalloids were isolated from chloroplasts using a continuous or a discontinuous Percoll gradient. The results obtained from chlorophyll (Chl) fluorescence emission spectra at 77 K indicated the presence of aggregates of light-harvesting complex proteins (LHC II) that was not connected to reaction centres of Photosystem II both in isolated chloroplasts and in the fraction of 80% Percoll gradient from both types of transgenic tobacco. Further analyses, i.e. pigmentcontents, polypeptide composition by SDS-PAGE, and immunoblotting support our hypothesis that crystalloids inside chloroplasts of transgenic tobacco are formed by LHC II aggregates. Treatment with two distinct detergents, chosen with ...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthetica

ISSN

0300-3604

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

509-517

Kód UT WoS článku

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EID výsledku v databázi Scopus

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