Transgenní ipt tabák s nadprodukcí cytokininů akumuluje fenolické látky během kultivace in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F06%3A00079985" target="_blank" >RIV/61389030:_____/06:00079985 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth

Popis výsledku v původním jazyce

We present evidence that overproduction of endogenous cytokinins caused stress response in non-rooting Pssu-ipt transgenic tobacco (Nicotiana tabacum L.) grown in vitro. It was demonstrated by overaccumulation of phenolic compounds, synthesis of pathogenesis related proteins, and increase in peroxidase activities. Immunolocalization of zeatin and also PR-1b protein on leaf cryosections proved their accumulation in all mesophyll cells of transgenic tobacco contrary to control non-transgenic plants. Intensive blue autofluorescence of phenolic compounds induced by UV in cross-sections of leaf midrib showed enhanced contents of phenolics in transgenic tobacco compared with controls, nevertheless, no significant difference between both plant types was foundin leaf total lignin content. Transgenic plantlets exhibited higher peroxidase activities of both soluble and ionically bound fractions compared with controls. HPLC analysis of phenolic acids confirmed the increase in all phenolic acids

Název v anglickém jazyce

Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth

Popis výsledku anglicky

We present evidence that overproduction of endogenous cytokinins caused stress response in non-rooting Pssu-ipt transgenic tobacco (Nicotiana tabacum L.) grown in vitro. It was demonstrated by overaccumulation of phenolic compounds, synthesis of pathogenesis related proteins, and increase in peroxidase activities. Immunolocalization of zeatin and also PR-1b protein on leaf cryosections proved their accumulation in all mesophyll cells of transgenic tobacco contrary to control non-transgenic plants. Intensive blue autofluorescence of phenolic compounds induced by UV in cross-sections of leaf midrib showed enhanced contents of phenolics in transgenic tobacco compared with controls, nevertheless, no significant difference between both plant types was foundin leaf total lignin content. Transgenic plantlets exhibited higher peroxidase activities of both soluble and ionically bound fractions compared with controls. HPLC analysis of phenolic acids confirmed the increase in all phenolic acids

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GA206%2F03%2F0310" target="_blank" >GA206/03/0310: Biotický stres způsobený virovou infekcí u transgenního Pssu-ipt tabáku</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

9

Strana od-do

526-534

Kód UT WoS článku

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EID výsledku v databázi Scopus

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