Auxin Immunolocalization Implicates Vesicular Neurotransmitter-Like Mode of Polar Auxin Transport in Root Apices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F06%3A00083353" target="_blank" >RIV/61389030:_____/06:00083353 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/06:00010625

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Auxin Immunolocalization Implicates Vesicular Neurotransmitter-Like Mode of Polar Auxin Transport in Root Apices

Popis výsledku v původním jazyce

Immunolocalization of auxin using a new specific antibody revealed, besides the expected diffuse cytoplasmic signal, the enrichment of auxin at cross-walls (end-poles, plant-synapses), within endosomes, and within nuclei of those root apex cells which accumulate abundant F-actin at their synapses. In Brefeldin A (BFA) treated roots, a strong auxin signal was scored within BFA compartments of cells having abundant actin and auxin at their synapses, as well as within adjacent endosomes, but not in other root cells. Interestingly, several types of polar auxin transport (PAT) inhibitors exert the same inhibitory effects on endocytosis, vesicle recycling, and on enrichment of F-actin at the plant-synapses. These findings indicate that auxin is secreted across F-actin-enriched plant synapses via neurotransmitter-like secretion. This new concept finds genetic support via the semaphore1, rum1, and rum1/lrt1 mutants of maize which are impaired in PAT, endocytosis and vesicle recycling, as well

Název v anglickém jazyce

Auxin Immunolocalization Implicates Vesicular Neurotransmitter-Like Mode of Polar Auxin Transport in Root Apices

Popis výsledku anglicky

Immunolocalization of auxin using a new specific antibody revealed, besides the expected diffuse cytoplasmic signal, the enrichment of auxin at cross-walls (end-poles, plant-synapses), within endosomes, and within nuclei of those root apex cells which accumulate abundant F-actin at their synapses. In Brefeldin A (BFA) treated roots, a strong auxin signal was scored within BFA compartments of cells having abundant actin and auxin at their synapses, as well as within adjacent endosomes, but not in other root cells. Interestingly, several types of polar auxin transport (PAT) inhibitors exert the same inhibitory effects on endocytosis, vesicle recycling, and on enrichment of F-actin at the plant-synapses. These findings indicate that auxin is secreted across F-actin-enriched plant synapses via neurotransmitter-like secretion. This new concept finds genetic support via the semaphore1, rum1, and rum1/lrt1 mutants of maize which are impaired in PAT, endocytosis and vesicle recycling, as well

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA301%2F05%2F0418" target="_blank" >GA301/05/0418: Nové generace inhibitorů cyklin-dependentních kinas s významnými protinádorovými účinky</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Signalling & Behavior

ISSN

1559-2316

e-ISSN

—

Svazek periodika

1

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

122-133

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—