Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F09%3A00336085" target="_blank" >RIV/61389030:_____/09:00336085 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

Popis výsledku v původním jazyce

Tobacco (Nicotiana tabacum L.) plantlets were grown on Murashige and Skoog medium in ventilated Magenta boxes and for the last subculture 10 A mu M ABA was added to the medium. After three weeks plantlets were transferred into pots with Perlite moistenedwith water and grown in controlled conditions (16-h photoperiod, day/night temperature 25/20 A degrees C, air humidity about 45 %) either under low or high irradiance of 150 (LI) and 700 (HI) A mu mol m(-2) s(-1), respectively. Content of endogenous ABAwas 271.7 pmol g(-1)(f.m.) in ABA treated plantlets, while in control plantlets it was only 53.3 pmol g(-1)(f.m.). After ex vitro transfer, stomatal conductance and transpiration rate decreased considerably in comparison with in vitro grown plantlets and remained lower also 7 d after ex vitro transfer, especially in ABA-treated plants and so wilting of plants was practically eliminated.

Název v anglickém jazyce

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

Popis výsledku anglicky

Tobacco (Nicotiana tabacum L.) plantlets were grown on Murashige and Skoog medium in ventilated Magenta boxes and for the last subculture 10 A mu M ABA was added to the medium. After three weeks plantlets were transferred into pots with Perlite moistenedwith water and grown in controlled conditions (16-h photoperiod, day/night temperature 25/20 A degrees C, air humidity about 45 %) either under low or high irradiance of 150 (LI) and 700 (HI) A mu mol m(-2) s(-1), respectively. Content of endogenous ABAwas 271.7 pmol g(-1)(f.m.) in ABA treated plantlets, while in control plantlets it was only 53.3 pmol g(-1)(f.m.). After ex vitro transfer, stomatal conductance and transpiration rate decreased considerably in comparison with in vitro grown plantlets and remained lower also 7 d after ex vitro transfer, especially in ABA-treated plants and so wilting of plants was practically eliminated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F07%2F0227" target="_blank" >GA522/07/0227: Fotoinhibice a oxidativní stres v průběhu aklimatizace in vitro pěstovaných rostlin</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Svazek periodika

53

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000272118600004

EID výsledku v databázi Scopus

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