AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F09%3A00337486" target="_blank" >RIV/61389030:_____/09:00337486 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis

Popis výsledku v původním jazyce

Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUSindicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.

Název v anglickém jazyce

AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis

Popis výsledku anglicky

Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUSindicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA206%2F06%2F1306" target="_blank" >GA206/06/1306: Úloha rovnováhy mezi cytokininy a kyselinou abscisovou při abiotickém stresu a během následného zotavení</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000272829800020

EID výsledku v databázi Scopus

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