Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F10%3A00347998" target="_blank" >RIV/61389030:_____/10:00347998 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

Popis výsledku v původním jazyce

The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing.

Název v anglickém jazyce

Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

Popis výsledku anglicky

The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Genomics

ISSN

1471-2164

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

122

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000275628900001

EID výsledku v databázi Scopus

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