Characterization of a new 4BS.7HL wheat?barley translocation line using GISH, FISH, and SSR markers and its effect on the ?-glucan content of wheat
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00366032" target="_blank" >RIV/61389030:_____/11:00366032 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1139/g11-044" target="_blank" >http://dx.doi.org/10.1139/g11-044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/g11-044" target="_blank" >10.1139/g11-044</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of a new 4BS.7HL wheat?barley translocation line using GISH, FISH, and SSR markers and its effect on the ?-glucan content of wheat
Popis výsledku v původním jazyce
A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat Asakaze komugi? barley Manas? hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitiveDNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-b-D-glucan production, was revealed in the centromeric region of 7HL.
Název v anglickém jazyce
Characterization of a new 4BS.7HL wheat?barley translocation line using GISH, FISH, and SSR markers and its effect on the ?-glucan content of wheat
Popis výsledku anglicky
A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat Asakaze komugi? barley Manas? hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitiveDNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-b-D-glucan production, was revealed in the centromeric region of 7HL.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Genome
ISSN
0831-2796
e-ISSN
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Svazek periodika
54
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
CA - Kanada
Počet stran výsledku
10
Strana od-do
795-804
Kód UT WoS článku
000296987100001
EID výsledku v databázi Scopus
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