Chromosome Analysis and Sorting Using Flow Cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00375427" target="_blank" >RIV/61389030:_____/11:00375427 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-61737-957-4_12" target="_blank" >http://dx.doi.org/10.1007/978-1-61737-957-4_12</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-61737-957-4_12" target="_blank" >10.1007/978-1-61737-957-4_12</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chromosome Analysis and Sorting Using Flow Cytometry

Popis výsledku v původním jazyce

Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure forpreparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can beinduced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications.

Název v anglickém jazyce

Chromosome Analysis and Sorting Using Flow Cytometry

Popis výsledku anglicky

Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure forpreparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can beinduced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Chromosome Engineering: Methods and Protocols

ISBN

978-1-61737-956-7

Počet stran výsledku

18

Strana od-do

221-237

Počet stran knihy

329

Název nakladatele

Humana Press

Místo vydání

New Jersey

Kód UT WoS kapitoly

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