Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00380694" target="_blank" >RIV/61389030:_____/12:00380694 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1439-0434.2012.01886.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2012.01886.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0434.2012.01886.x" target="_blank" >10.1111/j.1439-0434.2012.01886.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

Popis výsledku v původním jazyce

The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET-45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigenswere used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus.

Název v anglickém jazyce

Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

Popis výsledku anglicky

The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET-45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigenswere used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M06030" target="_blank" >1M06030: Funkční genomika a proteomika ve šlechtění rostlin</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology - Phytopathologische Zeitschrift

ISSN

0931-1785

e-ISSN

—

Svazek periodika

160

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

251-254

Kód UT WoS článku

000302465500007

EID výsledku v databázi Scopus

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